frozen tissue srorage

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From:Ian Montgomery <> (by way of histonet)
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>Date: Mon, 15 Nov 1999 12:14:03 -0600
>From: Sally Prouty <>
>Subject: frozen tissue srorage
>To: "Histonet Group (E-mail)" <>
>Thank you for your comments. Because of one of the comments I received I
>have another question. If the tissue samples are currently stored at -70 and
>I moved them to a -20 would I then get ice crystals due to the decrease in
>Sally Prouty
	If the initial freeze was good this shouldn't be a problem. Most tssues
are pretty tolerant of ice crystals, skeletal muscle being the exception.
I still wouldn't hold the tissue at -20 for extended periods.

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