Re: tissue processing

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From:"" <> (by way of histonet)
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Date sent:      	Wed, 17 Nov 1999 16:34:01 -0500
From:           	"Philopena, Jennifer" <>
Subject:        	tissue processing
To:             	"'HistoNet Server'" <>

> Hi.  I work with a Shandon Citadel 2000 and I (try to) process rat and mouse
> tissues (liver, lung, spleen, kidney, brain, heart).  The livers and spleens
> that I process are consistently very dry.

we use this processor for mouse tissues but only occasionally
have the problem of "dry" blocks (liver and spleen).I recently
presented a student with a project to assess the best processing
of various tissues, and to cut a long story short, the critical point
was fixation.After 12-18 hours in formalin we then trim the tissues
into cassettes and return to formalin for a further 4-5 hours, then
process from 70% alcohol (20 hours in total to wax). We found no
advantage in cutting down the processing time, in fact this was
often detrimental.The thing to remember is that although these
tissues are often small, morphologically they are the same as
human so it is important to slice livers, bisect spleen, kidney etc.
Hope this is of some help,

Terry Hacker,
Medical Research Council,
Oxfordshire, OX11 ORD
01235 834393 x360

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