Gayle,

I'm interested in the 1/2x coated slides you mentioned - up to what
size specimens of undecalcified bone have you  used these for?

Mary

>>> Gayle Callis <uvsgc@msu.oscs.montana.edu> - 11/18/1999 12:37 PM
>>>
I do not know the compatibility of Oil Red O and Crystalmount, I only
use
the Gentle Jane slides for lung/undecalcified bone frozens for IHC,
or Beta Gal stained whole tissue mounts. All our frozens for Oil Red
O,
and in general are just mounted on Plus charge slides, we reserve
the
Gentle Jane for very special protocols, not a routine everytime
usage.

I do have an Oil Red O protocol that eliminates the propylene glycol
as a solvent, is very easy, reliable and less messy.

I think it is important to not use 4X coated slides, even 1 X coated
slides, we successfully use the 1/2X coated slides even for
undecalcified
bone frozens, and this lessens the "bubble look" of the polymer after
is
is UV  polymerized.  Tried the 4X again, and immediately went to the
1/2X coated slides.

I do not notice the polymer when I do a normal dehydration, clearing
in
xylene, and a mounting media like Permount, but that is not
compatible
with the oil red o.

Love my Gentle Jane for the lung and bone thought, saves us load of
grief.

Gayle Callis