Re: RE: crystalmount/oil red O/gentle jane slides

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From:"Susan Meloan" <> (by way of histonet)
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I was also going to say that when used slides cut with the Instrumetics
tape, we got a background staining on the whole slide.  We usually mount
fat stains on plus slides also, and get a cleaner stain.

We have been  using the Shandon Immuno Mount.  I have wondered about
Crystal Mount, but have not tried it.


Susan Meloan
Chief Histotechnologist
Department of Pathology
Medical College of Georgia
Augusta, Ga 30912
(706) 721-3630

>>> "Tarpley, John" <> 11/18 2:42 PM >>>

> Gayle Callis wrote:
> Subject: 	crystalmount/oil red O/gentle jane slides
> I do not know the compatibility of Oil Red O and Crystalmount, I only use
> the Gentle Jane slides for lung/undecalcified bone frozens for IHC,
> or Beta Gal stained whole tissue mounts. All our frozens for Oil Red O,
> and in general are just mounted on Plus charge slides, we reserve the
> Gentle Jane for very special protocols, not a routine everytime usage.
Recently we had an occasion to section some difficult tissues for fat
staining and I tried the Instrumedics system. We use Sudan IV rather than
Oil Red O and we found it worked well with the stain. We mounted the
sections using Sandon's Immu-Mount water based mounting media. They didn't
look as clean as normal slides and did show the "bubble look" appearance,
but were still readable.
	-snip snip-
> I think it is important to not use 4X coated slides, even 1 X coated
> slides, we successfully use the 1/2X coated slides even for undecalcified
> bone frozens, and this lessens the "bubble look" of the polymer after is
> is UV  polymerized.  Tried the 4X again, and immediately went to the
> 1/2X coated slides.
I agree. We have the best results with 1/2x slides both for bone and soft

	-snip snip-

> John Tarpley 15-2-B
> Associate Scientist
> Specialist Image Analysis & Immunohistochemistry
> Amgen Inc
> One Amgen Center Drive
> Thousand Oaks, CA  91320
> Views expressed are mine alone and do not represent the views of my
> employer

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