We use Crystal Mount on oil red O, it works fine and
is a lot less messy.

Kathy Wortham
USDA, Food Safety Inspection Service
Athens, Ga


--- Susan Meloan <smeloan@mail.mcg.edu> wrote:
> John,
>
> I was also going to say that when used slides cut
> with the Instrumetics tape, we got a background
> staining on the whole slide.  We usually mount fat
> stains on plus slides also, and get a cleaner stain.
>
> We have been  using the Shandon Immuno Mount.  I
> have wondered about Crystal Mount, but have not
> tried it.
>
> Susan
>
> Susan Meloan
> Chief Histotechnologist
> Department of Pathology
> Medical College of Georgia
> Augusta, Ga 30912
> (706) 721-3630
> e-mail: smeloan@mail.mcg.edu
>
>
>
> >>> "Tarpley, John" <jtarpley@amgen.com> 11/18 2:42
> PM >>>
>
>
> > Gayle Callis wrote:
> > Subject: 	crystalmount/oil red O/gentle jane
> slides
> >
> > I do not know the compatibility of Oil Red O and
> Crystalmount, I only use
> > the Gentle Jane slides for lung/undecalcified bone
> frozens for IHC,
> > or Beta Gal stained whole tissue mounts. All our
> frozens for Oil Red O,
> > and in general are just mounted on Plus charge
> slides, we reserve the
> > Gentle Jane for very special protocols, not a
> routine everytime usage.
> Recently we had an occasion to section some
> difficult tissues for fat
> staining and I tried the Instrumedics system. We use
> Sudan IV rather than
> Oil Red O and we found it worked well with the
> stain. We mounted the
> sections using Sandon's Immu-Mount water based
> mounting media. They didn't
> look as clean as normal slides and did show the
> "bubble look" appearance,
> but were still readable.
> 	-snip snip-
> > I think it is important to not use 4X coated
> slides, even 1 X coated
> > slides, we successfully use the 1/2X coated slides
> even for undecalcified
> > bone frozens, and this lessens the "bubble look"
> of the polymer after is
> > is UV  polymerized.  Tried the 4X again, and
> immediately went to the
> > 1/2X coated slides.
> I agree. We have the best results with 1/2x slides
> both for bone and soft
> tissue.
>
> 	-snip snip-
>
> > John Tarpley 15-2-B
> > Associate Scientist
> > Specialist Image Analysis & Immunohistochemistry
> > Amgen Inc
> > One Amgen Center Drive
> > Thousand Oaks, CA  91320
> >
> > Views expressed are mine alone and do not
> represent the views of my
> > employer
>
>
>
>




=====
Kathy Wortham
USDA,Food Safety Inspection Service
950 College Station Road
Athens, Ga 30605
(706)546-3556
kjwortham@yahoo.com
__________________________________________________
Do You Yahoo!?
Bid and sell for free at http://auctions.yahoo.com