Re: Immuno staining
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Fri, 26 Nov 1999, Scott Schmitz wrote:
> We have been having trouble with our immunoperoxidase
> staining. The chromogen has been getting lighter and
> lighter in staining until finally today I had no staining.
My guess is that your stock of hydrogen peroxide
has deteriorated. The 30% solution has a way of
dying unpredictably. One cause is coming into
contact with almost any substance, including
phosphate ions and metals.
You can get a solid compound called urea hydrogen
peroxide that is more stable: You treat it as if
it were 35% and just weigh a bit out to make a
stock solution (1% or whatever) in water that you keep
in a clean glass bottle and change every week - or
every day if you want. UHP is greatly superior
to 30% hydrogen peroxide, and it seems to keep for
ever in the fridge. I've had the same jar for about
5 years.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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