Re: Biogenex Immunostainer

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From:Amos Brooks <> (by way of histonet)
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    I am unfamiliar with the Biogenex autostainer, but I am curious because
we have noticed occasional uneven staining on our DAKO
    Do the slides lie flat (which could indicate a leveling issue), or are
they held vertical using capillary action (which could
indicate a bad set of slides (spacers too far apart)).
    We are trying to rule out poor fixation on our controls right now.
Amos Brooks

Vicki L McKaughan wrote:

> We are currently demoing the Biogenex Immunostainer. Today we had a
>problem with the
> tissue not staining at the bottom of the slide.
> It sort of looked like the tissue did not get completely dewaxed.  We
>used the dewax on the
> instrument.  The tissue was very close to the
> barrier of the slide and I thought that perhaps
> this may have caused some pooling of solutions
> there.  We did a manual dewaxing yesterday and
> the slides were fine.  Has anyone else experienced problems with this?
>Also, has anyone had a problem with inconsistent staining
> due to the way the reagents are dispensed.  There appeared to be areas in
>which the reagents
> pooled on the slides as I was watching the
> instrument dispense and run.  Any comments or
> information would be greatly appreciated.
> Sincerely,
> Vicki McKaughan, HT Supervisor
> Camden Clark Memorial Hosptial
> Parkersburg, WV  26101
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