---------------------- Forwarded by Susannah G Winden/Surgery/mc/Duke on
11/24/99 11:32 AM ---------------------------


Susannah G Winden
11/24/99 09:53 AM

To:   Vicki L McKaughan <vlm_23@n2mail.com>
cc:

Subject:  Re: Biogenex Immunostainer  (Document link not converted)

Dear Vicki,

I am sure that you have spoken with  Biogenex technical service by now, but
here's my two cents worth just in case.  Try increasing the time and/ or
temperature that you heat your paraffin sections.  Although the EZ DeWax works
well, it works at a different rate then xylene and xylene substitute.
Therefore, the paraffin on your slides need to melt off as much as possible.
I was given this good advice from a Biogenex technical trainer.

I have not yet had trouble with reagents pooling at the bottom of the slide.
However, I have to double check to make sure my slides are laying flat in their
positions.  I found it hard to judge if the slide is flat on the black slide
rack.  So this could be your problem, unless all of you reagents are pooling on
your slides in the same way.  Then your Optimax could be sitting on an uneven
surface.  If none of this helps, then you could try to increase the amount of
reagents that are added to your individual slides.  This can be done on the
edit
screen, above the icons that show what part of the slide should be covered with
reagent.  Hope this helps!

Sincerely,
Susannah G. Winden, HT(ASCP)
Experimental Surgery Pathology Core Laboratory
Duke University Medical Center
Durham, NC






Vicki L McKaughan <vlm_23@n2mail.com> on 11/17/99 05:51:06 PM











 To:      histonet@pathology.swmed.edu

 cc:



 Subject: Biogenex Immunostainer







We are currently demoing the Biogenex Immunostainer. Today we had a problem
with
the
tissue not staining at the bottom of the slide.
It sort of looked like the tissue did not get completely dewaxed.  We used the
dewax on the
instrument.  The tissue was very close to the
barrier of the slide and I thought that perhaps
this may have caused some pooling of solutions
there.  We did a manual dewaxing yesterday and
the slides were fine.  Has anyone else experienced problems with this?  Also,
has anyone had a problem with inconsistent staining
due to the way the reagents are dispensed.  There appeared to be areas in which
the reagents
pooled on the slides as I was watching the
instrument dispense and run.  Any comments or
information would be greatly appreciated.

Sincerely,
Vicki McKaughan, HT Supervisor
Camden Clark Memorial Hosptial
Parkersburg, WV  26101
E-Mail...vlm_23@n2mail.com



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