The best way to do Immunoflourescence is on un-fixed tissue, but a fixative
called Michelles is excellent to hold the tissue , you must then wash tissue
in large volume
Malemide buffer, cut tissue in cryostat, then fix in cold acetone, then
stain. Stains come out gorgeous. I used this technique on Kidney, Liver, and
lymphnode for five years with great results. What tissue are you thinking of
doing? For what diagnosis?
                                        Dana Dittus
                                      Histology Supervisor
                                      Abington Hospital
                                   1-215-481-2609