RE: tissue processing (times; also clearing agents)
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From: | Cynthia Favara <cfavara@niaid.nih.gov> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
John,
Just a question in this regard. I attended a workshop about 4 years
ago and the information given was that specimens could be over dehydrated,
and that is why it is necessary to soak blocks. Now what I am apparently
hearing is that it is not enough dehydration [ I was going to say
underdehydration but don't know if that is a correct term] and this doesnot
allow for adequate penetration of the remaining reagents. So if this is
correct when a block has to be soaked inorder to be cut it is because the
specimen has not been adequately dehydrated and increasing the processing
time should help??? Have I got it right. Please let me know I would love to
be able to do rodent spleen and liver without having to soak.
Thanks,Cynthia Favara
NIAID/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286
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