RE: tissue processing (times; also clearing agents)

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From:Cynthia Favara <> (by way of histonet)
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	Just a question in this regard. I attended a workshop about 4 years
ago and the information given was that specimens could be over dehydrated,
and that is why it is necessary to soak blocks. Now what I am apparently
hearing is that it is not enough dehydration [ I was going to say
underdehydration but don't know if that is a correct term] and this doesnot
allow for adequate penetration of the remaining reagents. So if this is
correct when a block has to be soaked inorder to be cut it is because the
specimen has not been adequately dehydrated and increasing the processing
time should help??? Have I got it right. Please let me know I would love to
be able to do rodent spleen and liver without having to soak.
Thanks,Cynthia Favara
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286

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