RE: tissue processing

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From:Cynthia Favara <> (by way of histonet)
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	I use a Shandon Citadel 100 and do mostly mouse tissue. I have tried
numerous processing schedules and spleen andliver are the toughest. If I
shorten the times I get unprocessed specimens. this is what I do.
NBF 12-24 hrs
70% ETOH this is my first station and it is my variable set at 10 minutes
80% ETOH 40 min
95% ETOH 40 min
100% ETOH 30 min
100% ETOH 30 min
Propar 30 min
Propar 45 min
Propar 1 hr
Four wax changes with Sugipath infiltrating medium 30 minutes each the last
with vacuum. ope this is helpful.
Best of Luck and if you get something better please passs it on.
Cynthia Favara
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286

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