RE: tissue processing

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I too am having problems with my processing of mouse tissues.  I found that
my schedule of 15-20 min each step was too much and my tissues ended up
being really crunchy.  So at the NSH conference I talked to people who said
step down everything to 10 minutes or so and now my tissues are no properly
embedded and are more difficult to section than ever before plus the
tissues don't adhere well to silane slides, not to mention the H+E's look
awful .... HELP!!!


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