Jay:
We have injected specimens of the human wrist (from mid metacarpals
including the distal portion of the radius and ulna) with coloured latex in
the main vessels. The specimens were then left in 10 % NBF for fixation,
dehydrated in serial ethanols starting with 70 %, and cleared in methyl
salicylate. We have stored these undecalcified specimens in methylsalicylate
for a year or longer without deliterious effect.
Bancroft reports Spalteholz technique in the 4th ed of Theory and Practice
of Histological Techniques page 709 & 710.

Hope this helps, Donna Montague Orthopaedic Research UAMS Medical Center,
Little Rock, AR

-----Original Message-----
From: Jay Turner [mailto:turnerjayd@hotmail.com]
Sent: Monday, November 29, 1999 11:45 AM
To: histonet@pathology.swmed.edu
Subject: Spateholtz Solution


For demonstrating the intraosseous blood supply to the distal femur, I have
come across several protocols that inject with india ink and immerse with
"Spateholtz solution" to make the tissue transparent.  Is anyone familiar
with this technique?  What is Spateholtz solution and where can it be
purchased?

Thanks in advance.

Jay Turner
University of Medicine and Dentistry of New Jersey
Dept. of Orthopaedics
Newark, NJ

______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com