RE: Histochoice fixative

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From:Penelope Marr <MarrP@sesahs.nsw.GOV.AU> (by way of histonet)
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Hi Carla,
My experience with Histochoice was similar to Rob's.  The manufacturers
instructions say to leave tissue in Histochoice for a certain period of
time.  If the tissue is left for longer it autolyses.  After my
experiments I drew the conclusion that Histochoice stabilises certain
enzymes and antigens but it does not fix the tissue.  After processing
the tissue showed all the symptoms of fixation by whatever reagents it
was immersed in at the beginning of its processing schedule.

Penny Marr

South Eastern Sydney Area Health Service

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