Insitu pcr
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| From: | "Deborah Moffett" <DebMoffett@moffett.swinternet.co.uk> (by way of histonet) |
| To: | histonet@histosearch.com |
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| Content-Type: | text/plain; charset="us-ascii" |
We are attempting to do insitu PCR on gut and kidney paraffin sections for
bovine adenovirus. We are having some difficulty with disintegration of the
sections during the pcr cycles. Can anyone reccommend a solution to this
problem?
the sections are 4-5um thick , lifted on superfrost plus gold slides and
heated for one hour at 60C.
Deborah Moffett
Veterinary Sciences Division
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