Re: signal amplification methods

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From:Amos Brooks <> (by way of histonet)
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    DAKO has a CSA kit. CSA is short for Catalyzed Signal Amplification.
It is interesting technology. You can get staining of antigens that
normally would be lost after formalin fixation even after antigen
retrieval (e.g. cd4).
    Unfortunately the kit is very expensive, and a little complicated.
Call DAKO if you are interested. (DAKO    800 235-5763)
    Ventana has something similar too. It's much simpler, consisting of
one extra reagent. It's used on their NEXUS autostainer. I don't have
their information, but I'm sure someone on this list does.
Amos Brooks

Yuhui_Xu wrote:

> Dear All:
> I understand that currently there have been methods to amplify
> immunohistochemical signals, e.g.,TSA by DuPont. I would appreciate
> information
> about other methods in this direction.
> Thanks in advance.
> Yuhui

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