Re: cloudy film on back of slides

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From:Amos Brooks <> (by way of histonet)
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Hi Maureen,
    You probably are getting some residual water and alcohol in your Xylene.
Make sure the end alcohols are changed frequently, and use fresh Xylene to
coverslip with.
    If you are using a Xylene substitute it may still not be miscible in water,
and if it absorbs atmospheric water from the air you will get the precipitate
especially on the back of the slide. This happens very rapidly with acetone.
Amos Brooks
HT(ASCP) wrote:

> Hi Netters,
>     We have recently had a sporatic incidence of a cloudy, white film on the
> back side only of thin prep and medical gyn slides. It  does not  seem to
> interefere with reading the slide and it can only be removed by scraping it
> off with a razor knife. It only happens once in a while, usually when the
> reagents are fresh. Any ideas as to what is going on? Thank you.  Maureen

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