Re: big honkin' samples

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From:Tim Morken <> (by way of histonet)
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I've made stapeled lenspaper "bags" to process large tissue and "L" irons to
make blocks. You can use a 1" cubed wood block or a "histo-ring" plastic
embedding mount in the paraffin to make a clamp area. You will need to use
a screw clamp to hold it. the hard part is making sure the block or plastic
ring sits straight in the paraffin as it hardens.

Have fun!

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
1600 Clifton Rd.
Atlanta, GA 30333


Phone: (404) 639-3964
FAX:  (404)639-3043

----Original Message Follows----
From: Jeff Crews <>
Subject: big honkin' samples
Date: Fri, 12 Nov 1999 10:36:55 -0500

      Does anyone have any experience with embedding and sectioning
      large-cross-section tissues? We have some porcine vertebral samples
      that measure about  2" x 1 1/2". I can get larger cassettes for
      processing them and make some larger embedding molds, but how do you
      suggest that we clamp them in the microtome? This is the first time
      that we've had to do something this large, and I'd appreciate hearing
      from others about it. Thanks again!

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