Re: big honkin' samples
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From: | Tim Morken <timcdc@hotmail.com> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Jeff,
I've made stapeled lenspaper "bags" to process large tissue and "L" irons to
make blocks. You can use a 1" cubed wood block or a "histo-ring" plastic
embedding mount in the paraffin to make a clamp area. You will need to use
a screw clamp to hold it. the hard part is making sure the block or plastic
ring sits straight in the paraffin as it hardens.
Have fun!
Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA
email: tim9@cdc.gov
timcdc@hotmail.com
Phone: (404) 639-3964
FAX: (404)639-3043
----Original Message Follows----
From: Jeff Crews <jcrews@organo.com>
To: histonet@pathology.swmed.edu
Subject: big honkin' samples
Date: Fri, 12 Nov 1999 10:36:55 -0500
Does anyone have any experience with embedding and sectioning
large-cross-section tissues? We have some porcine vertebral samples
that measure about 2" x 1 1/2". I can get larger cassettes for
processing them and make some larger embedding molds, but how do you
suggest that we clamp them in the microtome? This is the first time
that we've had to do something this large, and I'd appreciate hearing
from others about it. Thanks again!
jc
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