Re: big honkin' samples
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From: | "P. Emry" <emry@u.washington.edu> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi Jeff,
I got a lot of help from others on the net when I started doing large pig
bones. I'll pass on what I have learned and used.
I had a fast-release chuck that slipped so that small bites were
being taken out of the specimens. I got one of the screw
down chucks which solved my problems and gave me the ability to use large
metal bases (aluminum with a grid) with mega-cassette sized blocks
attached that fit into the chuck. I inherited these things with the lab
so I don't know where you can buy them.
I also use a meg-cassette by filling it with paraffin. It fits nicely in
the screw down chuck. Unfortunately, the mega-cassette has a hole where
the hook on the lid fits. When you fill it you have to plug the hole
with bits of cooling paraffin or it leaks. I bought a glue gun and
plugged the hole on a second cassette I use for embedding.
(If there is a vendor listening, redesign the mega-cassette so that hole
is closed and won't let the paraffin leak out. Having to use two
cassettes per specimen is not a good thing and trying to plug it up with
cooling bits of paraffin is time consuming.)
Am I the only one with this problem or have you real "pros" found a way to
avoid this problem?
Best of luck.
Trisha
U of Washington, Seattle
On Fri, 12 Nov 1999, Jeff Crews wrote:
> Does anyone have any experience with embedding and sectioning
> large-cross-section tissues? We have some porcine vertebral samples
> that measure about 2" x 1 1/2". I can get larger cassettes for
> processing them and make some larger embedding molds, but how do you
> suggest that we clamp them in the microtome? This is the first time
> that we've had to do something this large, and I'd appreciate hearing
> from others about it. Thanks again!
> jc
>
>
>
Trisha
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