Re: TdT Testing
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From: | Dana Settembre <settembr@UMDNJ.EDU> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Tue, 9 Nov 1999, Daniel & Linda Botsford wrote:
> Do you have any recommendations for pretreatment or antibody supplier
> for TdT testing by immunoperoxidase on formalin fixed paraffin embeded
> tissue. We are currently have a Ventana Nexes stainer.
>
> Sincerely,
> Dan Botsford
> Windsor Regional Hospital
>
>
>
>
Hello Dan,
I use Dako's TdT catalog# A3524 at a 1:10 dilution with a thymus as a
control. I also use their LSAB2 kit as a detection system but I'm sure
other detection systems will work too. I pretreat with a citra microwave
step for 15 minutes. I'm sure that other pretreatments will work also.
Good Luck.
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark, New Jersey
USA
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