Re: TdT Testing

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From:Dana Settembre <settembr@UMDNJ.EDU> (by way of histonet)
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On Tue, 9 Nov 1999, Daniel & Linda Botsford wrote:

> Do you have any recommendations for pretreatment or antibody supplier
> for TdT testing by immunoperoxidase on formalin fixed paraffin embeded
> tissue. We are currently have a Ventana Nexes stainer.
> Sincerely,
> Dan Botsford
> Windsor Regional Hospital
Hello Dan,
I use Dako's TdT catalog# A3524  at a 1:10 dilution with a thymus as a
control.  I also use their LSAB2 kit as a detection system but I'm sure
other detection systems will work too.  I pretreat with a citra microwave
step for 15 minutes. I'm sure that other pretreatments will work also.
Good Luck.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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