Re: TdT Testing

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From:Amos Brooks <atbrooks@snet.net> (by way of histonet)
To:histonet@histosearch.com
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Hi Patst Ruegg
    I was wondering if you would have an address and/ or a phone number for
Super Tech. I've not heard of them that I can recall.
    How are their antibodies? We use DAKO detection kits as well. Which ones
do your doctors like better?
Amos Brooks

Patsy.Ruegg@UCHSC.edu wrote:

> we use the antibody from SuperTech at a 1:10 dilution with the decloaker
> pressure cooker for 20 min. in citrate buffer ph 6 hier pretreatment.  the
> detection is a vector quick kit LSAB hrp system.
> patsy ruegg
>
> -----Original Message-----
> From: Daniel & Linda Botsford [mailto:dl.botsford@sympatico.ca]
> Sent: Tuesday, November 09, 1999 5:10 AM
> To: HistoNet Server
> Subject: TdT Testing
>
> Do you have any recommendations for pretreatment or antibody supplier
> for TdT testing by immunoperoxidase on formalin fixed paraffin embeded
> tissue. We are currently have a Ventana Nexes stainer.
>
> Sincerely,
> Dan Botsford
> Windsor Regional Hospital




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