Re: Need Antigen Retrieval Help

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From:ThaxtonPM@aol.com (by way of histonet)
To:histonet@histosearch.com
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Hi Catherine,
  The easiest way to microwave AR is to watch the coplin jar while
microwaving on high heat. When you see the solution bubble, stop the
microwave and check the temperature (also record the time that it took to get
to temp). Microwave again on medium heat, and check the temperature at each
interval to make sure the temperature is holding. It is best to do this using
water to begin with, and always make sure you have the same number of slides
in the coplin jar each time.
Once you get the procedure standardized with the microwave you are using, you
should be ok.


okay, back in my corner now,
        Phyllis




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