Dear Lynn,

> Hey Guys, have a question for all of you doing Immunohistochemistry on
>formalin fixed paraffin embedded tissues.
>
> Has anyone out in histoland counterstained this type of Immuno with PAS
>or Harris Hematoxylin and Eosin?

Yes I have. You can do the routine IPX stain with DAB, followed by
PAT (Periodic Acid Thionin). This should give you the same result as
a PAS.
Or you can use cobalt to intensify and change the colour of
the brown DAB to Blue and follow it with a PAS.

You can use a H&E counterstain, though be sure that the eosin is weak
(eg 1% aqueous eosin - no acid or other red dyes eg phloxine). You
may need to intensify the DAB with (Oh NO) osmium or you could use
amoniacal silver.

Hope this helps,  Tony
.
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA

http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html