Re: Haematoxylin

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From:Tony Henwood <> (by way of histonet)
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Dear Andre,

> Some time ago I saw a recipe for Mayers Haematoxylin, on the
> Histonet,where in stead of Chloral Hydrate you use Glycerine.  I
> know that I printed that one but I cannot find it.  Can someone help?
> Thanks in advance.

The following stain may be of use:

 NOTES: This solution is a progressive haematoxylin that uses 10%
less alum. Chloral hydrate, because of its toxicity, has been
replaced with ethanol, a more effective penetrator and bacteriostat.
Mercury oxide has been replaced with sodium iodite.
Dissolve 45g ammonium or potassium alum in 900ml distilled water with
the aid of heat. Dissolve 2.5g Haematoxylin  in100ml absolute
ethanol. Combine above solutions and add 0.2g Sodium Iodite and 1g
citric acid. Mix well. The solution is stable for several months.
1.	Dewax and hydrate sections.
2.	Stain in modified Mayer's Haematoxylin 40secs.
3.	Wash in warm water 5min.
4.	If required, counterstain in eosin solution.
5.	Dehydrate, clear and mount.

Garvie, W., (1991) J. Histotechn 14(3):164-165.

Regards, Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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