Hi,
    What detection kit are you using? We use a pre diluted DAKO
antibody, with the LSAB2 system (30 min primary, 10 min secondary, and
10 min tertiary) with 20 min HIER epitope retrieval.
good luck
Amos Brooks
HT(ASCP)

joyce judge wrote:

> I am looking for a good antibody and protocol for
> HMBR45(Melanoma).
>
> Joyce Judge
> Saints Memorial Medical Center
>
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