Re: Glycoprotein IIIa (CD61)

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From:Amos Brooks <> (by way of histonet)
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Hello Nancy Shellhorn
    We at DIANON get good results with the antibody Glycoprotein IIIa,CD61
from DAKO (catalog #N1571).
    We use heat induced epitope retrieval (HIER), for 20 min. Then 30 min in
primary antibody, 10 min in secondary, 10 min tertiary using the LSAB2 kit.
good luck,
let me know how you do.
Amos Brooks

Nancy Shellhorn wrote:

> Hi Histonetters,
> Does anyone out there have any experience working with this antibody?  I
> have a friend that gets it to work great in smears but has absolutely no
> luck on bone marrow biopsies in paraffin.  Any help would be appreciated!
> Thanks a lot!
> Nancy
> Nancy Shellhorn
> DAKO Corporation
> 1-800-400-DAKO ext. #5385
> Fax: 904-249-6441

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