Re: FISH procedures and theory etc...

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Fish is a molecular technique with some properties similiar to Ihc ,but a
higher sensitivity level, the protocols are trickier and are even more
precise, this method has a flourescent tag or label so special filters are
needed to read it on a microscope.
It can be frustrating depending on which probe you are looking at and for.
There are methods that are labeled with chromogens, and they can be tricky
too, for example a Ish technique on liver could have problems if the tag is
biotin. This is the tip, of the iecberg only,but certainly the wave of the
                                                     Dana Dittus
                                                Abington Memorial Hospital
                                               Histology Supervisor

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