Has anyone else had the situation occur in which good nickel-enhanced DAB
(either fresh or out-of-date) vanishes or decreases after
alcohols/clearing? We have had some runs where I would swear that this
had happened; it looked good during the reaction, but after clearing the
staining was significantly reduced. Just how permanent is DAB anyway, and
under what conditions can you get it to wash out like this? I'd love to
hear from you all on this. thanks.
							jc

On Thu, 11 Nov 1999 09:25:42 +0000 Ian Montgomery
<ian.montgomery@bio.gla.ac.uk> writes:
>	My question yesterday regarding DAB was just to gauge reaction
>as to old
>DAB kits. I hadn't noticed, but my kit expired last April and until
>recently had worked fine. Yesterday I popped through some sections in
>the
>normal way and checked the DAB reaction with a microscope. Beautiful
>result, clean as a whistle and good and black (nickel enhanced).
>After
>counterstaining and DCM the reaction product had gone, vanished like
>'snaw
>aff a dyke'. From comments received I think I will just buy fresh and
>pay
>more attention to expiry dates.
>Ian.
>
>

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