Hi Dana,
I agree with you that the proteolytic digestion generally works well
with cytokeratins, but I also agree with Amos on the high molecular
weight cytokeratin (*clone 34Be12) turning out absolutely best with
HIER. I've tried pepsin, trypsin and pronase E and there is no
comparison. On the other hand, cytokeratin Cam 5.2 is most brilliant
with pronase E. It seems, to get optimal results, each antibody needs to
be tested with various retrievals or no retrieval. My 2 cents worth...

Katri

Katri Tuomala
Anatomic Pathology
St.Joseph's Hospital
Hamilton, Ontario, Canada