Re: Ck7 & Ck20

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From:Amos Brooks <atbrooks@snet.net> (by way of histonet)
To:histonet@histosearch.com
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HI,
   ck7 1:50 ( m7018 ) and ck20 1:100 ( m7019 )  this is from DAKO. Target
retrieve them using heat induced epitope retrieval. Then incubate for 30
min. in primary , secondary and tertiary  LSAB+ detection kit from DAKO.
This usually works quite nicely.
    gee does it look like I use DAKO junk occasionally
good luck.
Amos

Muhammad Tahseen wrote:

> Could some tell me a good CK7 and CK20 antibody  for formalin-fixed
> paraffin embedded tissues.  What procedures and pretreatments are you
> using.
> Any suggestions would be appreciated.
> Thank you very much in advance!
>
> Muhammad Tahseen
> Histology Supervisor,
> SKMT&RC.
> Lahore,Pakistan.
> tahseen@brain.net.pk




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