Re: Ck7 & Ck20
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From: | Dana Settembre <settembr@UMDNJ.EDU> (by way of histonet) |
To: | histonet@histosearch.com |
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Content-Type: | text/plain; charset="us-ascii" |
On Thu, 11 Nov 1999, Muhammad Tahseen wrote:
> Could some tell me a good CK7 and CK20 antibody for formalin-fixed
> paraffin embedded tissues. What procedures and pretreatments are you
> using.
> Any suggestions would be appreciated.
> Thank you very much in advance!
>
> Muhammad Tahseen
> Histology Supervisor,
> SKMT&RC.
> Lahore,Pakistan.
> tahseen@brain.net.pk
>
>
>
>
Hello Muhammad,
I use Dako's Cytokeratin 7 cat# M7018 with Dako's Prot. K at room temp for
5 minutes and then the antibody at a 1:800 dilution incubating for 15
minutes room temp. I use their Cytokeratin 20 cat# M7019 with a citra
buffer pretreatment in the microwave for 15minutes and incubate for
15minutes at room temp. too. I also use their LSAB2 detection kit, but
other detection kits work too.
Good Luck
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark, New Jersey
USA
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