Re: Ck7 & Ck20

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From:Dana Settembre <settembr@UMDNJ.EDU> (by way of histonet)
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On Thu, 11 Nov 1999, Muhammad Tahseen wrote:

> Could some tell me a good CK7 and CK20 antibody  for formalin-fixed
> paraffin embedded tissues.  What procedures and pretreatments are you
> using.
> Any suggestions would be appreciated.
> Thank you very much in advance!
> Muhammad Tahseen
> Histology Supervisor,
> Lahore,Pakistan.
Hello Muhammad,
I use Dako's Cytokeratin 7 cat# M7018 with Dako's Prot. K at room temp for
5 minutes and then the antibody at a 1:800 dilution incubating for 15
minutes room temp.  I use their Cytokeratin 20 cat# M7019 with a citra
buffer pretreatment in the microwave for 15minutes and incubate for
15minutes at room temp. too.  I also use their LSAB2 detection kit, but
other detection kits work too.
Good Luck

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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