Re: Calretinin on cytospins revisisted -Reply

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From:"Richard Cartun" <> (by way of histonet)
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Do you really need to heat-treat specimens that are not fixed in formalin?
Maybe the gorgeous immunoreactivity you speak of is due to the 37 degree
primary antibody incubation.  I would appreciate hearing from the Histonet
community regarding this issue (should non-formalin-fixed specimens be
heat-treated for optimal immunoreactivity?).

R. Cartun

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