Re: Calretinin on cytospins revisisted -Reply
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| From: | "Richard Cartun" <Rcartun@harthosp.org> (by way of histonet) |
| To: | histonet@histosearch.com |
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| Content-Type: | text/plain; charset="us-ascii" |
Do you really need to heat-treat specimens that are not fixed in formalin?
Maybe the gorgeous immunoreactivity you speak of is due to the 37 degree
primary antibody incubation. I would appreciate hearing from the Histonet
community regarding this issue (should non-formalin-fixed specimens be
heat-treated for optimal immunoreactivity?).
R. Cartun
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