Re: CD31,CD34

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From:Barbara Wright <> (by way of histonet)
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huCD34 does not cross react with mouse.  Pharmingen has a rat anti-mouse
CD34 (Clone RAM34) that works on frozen sections and a rat anti-mouse CD31
(Clone MEC13.3) that works on both frozen and paraffin mouse tissues.
muCD31 works on 10%NBF, PLP and 4% Paraformaldehyde fixed paraffin embedded
mouse kidney with high ph citra (AR10 from Biogenex) antigen retrieval with
tyramide amplification (TSA from NEN).  For most CD markers they are
specific for the species intended especially if they are monoclonal
antibodies.  You may have some luck with with cross reactivity with
polyclonals (e.g.. rabbit anti-human CD3 does work on rodents).

Lee Ann Baldridge wrote:

> Hi All,
> I'm having big time trouble getting CD34 to stain in mouse kidney.  I
>used Dako's primary and Vector's MOM. No staining. Also no staining with
>the Pharmingen's CD31(rat anti-mouse) in tumor cells implanted in a mouse
>brain.  I tried Dako's LSAB2 kit and Vector's MOM just for kicks.  Any
>help or suggestions would be greatly appriecated. I'm new at this animal
>tissue stuff so be patient with my frequent questions. Thanks for your
> Lee Ann Baldridge
> I.U. Hosp.
> Indpls., IN.
> 317-274-5790
> 317-278-4820 fax

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