RE: question on a paraffin product

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From:"P. Emry" <emry@u.washington.edu> (by way of histonet)
To:histonet@histosearch.com
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Hi,

Surgipath has a new paraffin "R" I think it is called.
I contacted them and was advised to do both processing and embedding with
it. I am doing decalcified bone specimens.  I just do 2 para...3rd under
vacuum.  Would you (all) suggest doing more?
I just started cutting the blocks and am having some wrinkling problems.
With these specimens, curved brain case sections I might have this problem
with any para.  Pain!  Any suggestions?

Trisha
U/W Seattle

On Tue, 2 Nov 1999, MacDonald, Jennifer wrote:

>
>
> 	Gayle:
>
> 	We are using both the infiltrating and the embedding media from
> Surgipath.  We have tried a few others but keep coming back to the two
> paraffin system.
>
> 	Jennifer
>
> > Has anyone used the Surgipath embedding media (part of their two paraffin
> > system- one for infiltration with less polymer, one for embedding with
> > poymer) for infiltration.  I am about to embark on this, mainly since
> > I have extra embedding media, a VIP processor with vacuum/pressure
> > options,
> > to aid in the infiltration.
> >
> > I can't think why it wouldn't work, may even improve sectioning.  Maybe
> > someone from Surgipath will enter discussion.  Any comments!
> >
> > Gayle Callis
> >
> >
> >
>
>

Trisha




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