Linda,

if you have a fluorescent microscopy available, what about inducing
fluorescence of the aromatic amines within the ganglia? There is a
reference in the 4th edition of Bancroft (Eranko, 1955).  This could be
completed in 5 to 10 minutes.

Rob

Robert S. Geske
Research Associate
Center for Comparative Medicine and Department of Pediatrics
Baylor College of Medicine

-----Original Message-----
From:	Sebree Linda A. [SMTP:la.sebree@HOSP.WISC.EDU]
Sent:	Wednesday, September 01, 1999 2:04 PM
To:	'Histonet'; 'IPOX'
Subject:	ganglia identification in colonoscopy biopsies

Hi Histonetters and IPOXers,

One of our pathologists has asked us to find a way to identify the presence
of ganglia in frozen sections of colon biopsies.  Colonoscopy is performed
to identify Hirschsprung's disease.  The idea is to sample sections of
colon
and perform quick stains to access the presence or absence of ganglia.  I
don't know of any immunohistochemistry procedure that can be done within
the
30-45 minutes time frame.  All of the biopsies would be assessed while the
patient is on the O.R. table.

So....does anyone out there know of a histochemical, enzyme or
immunohistochemical procedure that could be used?

Your help, as always, is greatly appreciated,

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI  53792-2472


(608)265-6596
FAX: (608)263-1568


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