we use the antibody from SuperTech at a 1:10 dilution with the decloaker
pressure cooker for 20 min. in citrate buffer ph 6 hier pretreatment.  the
detection is a vector quick kit LSAB hrp system.
patsy ruegg

-----Original Message-----
From: Daniel & Linda Botsford [mailto:dl.botsford@sympatico.ca]
Sent: Tuesday, November 09, 1999 5:10 AM
To: HistoNet Server
Subject: TdT Testing


Do you have any recommendations for pretreatment or antibody supplier
for TdT testing by immunoperoxidase on formalin fixed paraffin embeded
tissue. We are currently have a Ventana Nexes stainer.

Sincerely,
Dan Botsford
Windsor Regional Hospital