RE: IHC counterstain

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From:"Patterson, Noelle" <PattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL> (by way of histonet)
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Eosin tinges the DAB a very pinkish (read pale/golden) brown.  The contrast
is poor, so eosin is generally not recommended.  A bit off of your question.
I would be very interested if PAS works after immuno without altering the
immuno staining pattern.


> ----------
> From: 	Lynn Gardner[]
> Sent: 	Tuesday, November 09, 1999 9:23 AM
> To:
> Hey Guys, have a question for all of you doing Immunohistochemistry on
> formalin fixed paraffin embedded tissues.
> Has anyone out in histoland counterstained this type of Immuno with PAS or
> Harris Hematoxylin and Eosin?
> Your help is greatly appreciated!
> Lynn Gardner

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