RE: IHC counterstain
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From: | "Patterson, Noelle" <PattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL> (by way of histonet) |
To: | histonet@histosearch.com |
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Content-Type: | text/plain; charset="us-ascii" |
Eosin tinges the DAB a very pinkish (read pale/golden) brown. The contrast
is poor, so eosin is generally not recommended. A bit off of your question.
I would be very interested if PAS works after immuno without altering the
immuno staining pattern.
Noelle
> ----------
> From: Lynn Gardner[SMTP:lynn-gardner@uiowa.edu]
> Sent: Tuesday, November 09, 1999 9:23 AM
> To: histonet@pathology.swmed.edu
>
> Hey Guys, have a question for all of you doing Immunohistochemistry on
> formalin fixed paraffin embedded tissues.
>
> Has anyone out in histoland counterstained this type of Immuno with PAS or
> Harris Hematoxylin and Eosin?
>
> Your help is greatly appreciated!
> Lynn Gardner
>
>
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