RE: Daily Digest

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From:"Goolsby, Holly" <HGOOLSBY@PARTNERS.ORG> (by way of histonet)
To:histonet@histosearch.com
Reply-To:
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Hi,
Will immuno staining be consistant between species?  Owl monkey brain stains
beautifully with L26, HAM56, and CD3.
However Marmoset brain stained with the same antibodies at the same
dilutions and protocols shows no staining.  Can anyone shed any light on
this?

Many thanks,
Holly Goolsby
Neuropathology, Massachusetts General Hospital
hgoolsby@partners.org

> -----Original Message-----
> From:	HistoNet Server [SMTP:histonet@pathology.swmed.edu]
> Sent:	Friday, November 05, 1999 1:12 AM
> To:	HistoNet Server
> Subject:	Daily Digest
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 06:15:45 -0600
> From: dl.botsford@sympatico.ca
> Subject: Attention Tissue Processor Venders
>
> Hi,
> We are suddenly in the market for a tissue processor. I have contacted
> the following companies: Bayer,Esbe, Fisher,Leica,Somagen, Ventana. If
> there are any other venders selling tissue processors please contact me.
> I need capacity, bench space needed and pricing in Canadian Dollars.
>
> Sincerely,
> Dan Botsford
> 1995 Lens Avenue,
> Windsor Ontario,Canada
> N8W 1L9
> 519-254-5577 ext.52245
> 519-254-6861 FAX
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 06:16:11 -0600
> From: dl.botsford@sympatico.ca
> Subject: Tissue Processor-Users Survey
>
> We are suddenly in the market for a new tissue processor.I would
> appreciate you sharing your experiences about your machine. Are there
> any drawbacks or particular advantages to the machine you purchased?
>
> Sincerely,
> Dan Botsford
> Windsor Regional Hospital
> Windsor , Ontario, Canada
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 06:16:36 -0600
> From: "Rae Staskiewicz" <raestask@galesburg.net>
> Subject: Re: tonsils
>
> Kevin,
>
>     Yes, pigs have tonsils. We routinely section and stain them for the
> presence of circo virus.
>
> Rae Staskiewicz, HT (ASCP)
> Galesburg Animal Disease Lab
> Galesburg, IL
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 06:59:42 -0600
> From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
> Subject: RE: tonsils
>
> Yes indeed, pigs do have tonsils and they contain quite a collection of
> assorted bacteria and viruses.
> Swabbing a pigs tonsils is a challenge for obtaining cultures. They also
> contribute to their distinctive snort but,
> they do not affect the way they fly.
>
>
>
> Eric C. Kellar
> University of Pittsburgh Medical Center
>
>
> > ----------
> > From: 	kevin gibbon[SMTP:gibbowax@uniserve.com]
> > Sent: 	Wednesday, November 03, 1999 11:27 PM
> > To: 	Histonet@pathology.swmed.edu
> > Subject: 	tonsils
> >
> > Hi everyone,
> > 	someone asked me a couple of weeks ago if pigs had tonsils, I don't
> > know
> > so I wondered if anyone out in Histoland knows for definite if they do
> or
> > they don't.
> >
> > Thanks
> >
> > Kevin Gibbon
> > Wax-it Histology Services
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 07:02:21 -0600
> From: "Tim Morken" <timcdc@hotmail.com>
> Subject: Re: label printers
>
> Cynthia,
>
> We use Nalgene laser labels. They can be fed through a laser printer as
> many
> times as you want with no harm to the labels or the printer. The only
> drawback is that the labels on the edge of the sheet are too close to
> print
> on with most laser printers. that means that you can only use 54 of 88
> labels per sheet. Still, they are the best laser labels I have found. The
> following is the Fisher ordering info but you won't find these in the
> Fisher
> catalog.
>
> Nalgene labels (Labels, Histology Slide, Nalgene Polypaper, Fisher
> Scientific, CAT# 13-382-87A, 7/8" x 7/8", 88 labels per sheet, 10 sheets
> per
> pack, $25/pk. these are designed for the laser printer.
>
>
> Tim Morken, B.A., EMT(MSA), HTL(ASCP)
> Infectious Disease Pathology
> Centers for Disease Control
> MS-G32
> 1600 Clifton Rd.
> Atlanta, GA 30333
> USA
>
> email: tim9@cdc.gov
>        timcdc@hotmail.com
>
> Phone: (404) 639-3964
> FAX:  (404)639-3043
>
>
>
> - ----Original Message Follows----
> From: Cynthia Favara <cfavara@niaid.nih.gov>
> To: "'HistoNet Server'" <HistoNet@pathology.swmed.edu>
> Subject: label printers
> Date: Wed, 3 Nov 1999 17:39:03 -0500
>
> Does anyone have a label printer that can use Word Perfect that works
> well.
> We currently use a laser printer and wast a lot of labels trying to refeed
> a
> sheet. Thanks
> Cynthia Favara
> NIAID/RML/LPVD
> 903 South 4th Street
> Hamilton, MT 59840
> PH: 406-363-9317
> FAX: 406-363-9286
>
>
>
>
>
>
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 07:48:16 -0600
> From: Bernie Curran <Bernie.Curran@amnch.ie>
> Subject: Antibodies to Inhibitor 2 of Protein Phosphatase 1??
>
> From:		Bernie Curran, Cellular Pathology Lab., Tallaght Hospital,
> Dublin 24, Ireland.
>
> Message:	Does anyone know a source for antibodies to the Inhibitor 2
> of Protein 			Phosphatase 1 (IPP2)		Thanks
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 07:48:47 -0600
> From: "rgeske@bcm.tmc.edu" <rgeske@bcm.tmc.edu>
> Subject: RE: ganglia identification in colonoscopy biopsies
>
> Linda,
>
> if you have a fluorescent microscopy available, what about inducing
> fluorescence of the aromatic amines within the ganglia? There is a
> reference in the 4th edition of Bancroft (Eranko, 1955).  This could be
> completed in 5 to 10 minutes.
>
> Rob
>
> Robert S. Geske
> Research Associate
> Center for Comparative Medicine and Department of Pediatrics
> Baylor College of Medicine
>
> - -----Original Message-----
> From:	Sebree Linda A. [SMTP:la.sebree@HOSP.WISC.EDU]
> Sent:	Wednesday, September 01, 1999 2:04 PM
> To:	'Histonet'; 'IPOX'
> Subject:	ganglia identification in colonoscopy biopsies
>
> Hi Histonetters and IPOXers,
>
> One of our pathologists has asked us to find a way to identify the
> presence
> of ganglia in frozen sections of colon biopsies.  Colonoscopy is performed
> to identify Hirschsprung's disease.  The idea is to sample sections of
> colon
> and perform quick stains to access the presence or absence of ganglia.  I
> don't know of any immunohistochemistry procedure that can be done within
> the
> 30-45 minutes time frame.  All of the biopsies would be assessed while the
> patient is on the O.R. table.
>
> So....does anyone out there know of a histochemical, enzyme or
> immunohistochemical procedure that could be used?
>
> Your help, as always, is greatly appreciated,
>
> Linda A. Sebree, HT
> University of Wisconsin Hospital & Clinics
> Immunohistochemistry/In Situ Hybridization Laboratory
> D4/218-2472
> 600 Highland Avenue
> Madison, WI  53792-2472
>
>
> (608)265-6596
> FAX: (608)263-1568
>
>
> - ------------------------------------------------------------------------
>
> - ----
> This message was posted through the Stanford campus mailing list server.
>  If
> If you wish to unsubscribe from this mailing list, email the message body
> of "unsubscribe ipox-l" to majordomo@pathology.stanford.edu.
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 07:49:12 -0600
> From: "rgeske@bcm.tmc.edu" <rgeske@bcm.tmc.edu>
> Subject: Recall: ganglia identification in colonoscopy biopsies
>
> Rob Geske would like to recall the message, "ganglia identification in
> colonoscopy biopsies".
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 08:31:21 -0600
> From: kkdulany@unmc.edu
> Subject: Re: Tissue Processor-Users Survey
>
>
> In my opinion the VIP tissue processor is wonderful.  We have had ours for
> several years and never any problems.  Easy to run and very reliable.
> Karen Dulany HTL (ASCP)
> Eppley Institute for Cancer Research
> Omaha, NE
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 08:49:14 -0600
> From: "Bruce W. Brodersen" <bbrodersen1@unl.edu>
> Subject: RE: tonsils
>
> Yup!
>
> Bruce W. Brodersen, DVM, PhD   Email:  bbrodersen1@unl.edu
> University of Nebraska         Voice:  402 472-1434
> Veterinary Diagnostic Center   FAX:    402 472-3094
> Lincoln, NE  68583-0907
>
> - -----Original Message-----
> From: kevin gibbon [mailto:gibbowax@uniserve.com]
> Sent: Wednesday, November 03, 1999 11:09 PM
> To: Histonet@pathology.swmed.edu
> Subject: tonsils
>
>
>
>
> Hi everyone,
>         someone asked me a couple of weeks ago if pigs had
> tonsils, I don't
> know
> so I wondered if anyone out in Histoland knows for definite if
> they do or
> they don't.
>
> Thanks
>
> Kevin Gibbon
> Wax-it Histology Services
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 08:49:40 -0600
> From: DODI BORSAY HOROWITZ <BORSAY.DODI@epamail.epa.gov> (DODI BORSAY)
> Subject: Pathologist's Assistants Programs
>
> Try this URL link for the homepages of American Association of
> Pathologists' Assistants:
>
> http://www.pathologistsassistants.org/
>
> Links to training programs, regulations, programs, meetings, jobs,
> etc.....
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 08:50:06 -0600
> From: gossg@po.lab.ccf.org
> Subject: Re: pathology assistant schools
>
> Call the American Association for Pathology Assistants for info 1-800-
> 532-
> AAPA
>
> On Wed, 3 Nov 1999, NEVADUNNE@aol.com wrote:
> >Hey Netters,
> >    Can anyone tell me if there are programs for pathology assistants
> >available in the Baltimore or Philadelphia area? I am a histotech who is
> >currently doing practically all the gross dissection for our lab. I would
>
> >like to get certification, or possibly be "grandfathered" as a pathology
> >assistant. Any help would be appreciated.  Thanks.
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 09:45:30 -0600
> From: Histonet <Histonet@dakousa.com>
> Subject: RE: Attention Tissue Processor Venders
>
> Dear Dan,
>
> Sakura sells Tissue-tek VIP Tissue Processor.  Feel free to contact Bob
> Weingard at Sakura, phone 310-972-7871 or email him at
> bob.weingard@sakuraus.com
>
> Sincerely,
> Danielle McCombs
> DAKO Corporation
> Technical Service Specialist
> email danielle.mccombs@dakousa.com
> (800)235-5743 ext. 5321
>
> - -----Original Message-----
> From: Daniel & Linda Botsford [mailto:dl.botsford@sympatico.ca]
> Sent: Thursday, November 04, 1999 4:07 AM
> To: HistoNet Server
> Subject: Attention Tissue Processor Venders
>
>
> Hi,
> We are suddenly in the market for a tissue processor. I have contacted
> the following companies: Bayer,Esbe, Fisher,Leica,Somagen, Ventana. If
> there are any other venders selling tissue processors please contact me.
> I need capacity, bench space needed and pricing in Canadian Dollars.
>
> Sincerely,
> Dan Botsford
> 1995 Lens Avenue,
> Windsor Ontario,Canada
> N8W 1L9
> 519-254-5577 ext.52245
> 519-254-6861 FAX
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 09:45:57 -0600
> From: Histonet <Histonet@dakousa.com>
> Subject: RE: HMBR45
>
> Dear Joyce,
>
> Dako carries HMB45, product code M0634.  I can fax you the specification
> sheet and protocol information.  Please feel free to contact me.
>
> Sincerely,
> Danielle McCombs
> DAKO Corporation
> Technical Service Specialist
> email danielle.mccombs@dakousa.com
> (800)424-0021 ext. 5321
>
> - -----Original Message-----
> From: joyce judge [mailto:joycejudge@yahoo.com]
> Sent: Wednesday, November 03, 1999 4:58 PM
> To: histonet@pathology.swmed.edu
> Subject: HMBR45
>
>
> I am looking for a good antibody and protocol for
> HMBR45(Melanoma).
>
> Joyce Judge
> Saints Memorial Medical Center
>
> =====
>
> __________________________________________________
> Do You Yahoo!?
> Bid and sell for free at http://auctions.yahoo.com
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 10:19:11 -0600
> From: "Richard Cartun" <Rcartun@harthosp.org>
> Subject: Re: Calretinin on cytospins revisisted -Reply
>
> Do you really need to heat-treat specimens that are not fixed in formalin?
>
> Maybe the gorgeous immunoreactivity you speak of is due to the 37 degree
> primary antibody incubation.  I would appreciate hearing from the Histonet
> community regarding this issue (should non-formalin-fixed specimens be
> heat-treated for optimal immunoreactivity?).
>
> R. Cartun
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 10:39:57 -0600
> From: Gary Radice <gradice@richmond.edu>
> Subject: characterizing cartilage
>
> I have a begun a collaboration with a colleague (a surgeon) who is
> interested in cartilage regeneration. My job is  to determine whether the
> regenerated tissue it looks (histologically) like "real" articular
> cartilage. Most of my experience is with embryonic tissues and with
> muscle.
> I have checked with all the usual suspects (Humason's Animal Tissue
> Techniques, Bancroft and Stevens, AFIP manuals) for standard techniques
> but
> I would also like to correspond with someone who does cartilage routinely
> to get some opinions about protocols that might be particularly diagnostic
> for "normality."
>
> Gary P. Radice			gradice@richmond.edu
> Associate Professor of Biology		804 289 8107 (voice)
> University of Richmond		804 289 8233 (FAX)
> Richmond VA 23173		http://www.science.richmond.edu/~radice
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 10:57:24 -0600
> From: "Liz Standish" <LSTANDISH@govsci.com>
> Subject:
>
> I am trying to find out who manufacturers accra gill hematoxalin II and
> where it can be purchased. Thanks
> Liz Standish
> GSS
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 11:31:15 -0600
> From: "Goodwin, Diana" <DGoodwin@CHSNJ.org>
> Subject: CPT code for H. pylori screens
>
> Hello, Netters.
>
> Does anyone know if there is a CPT code for billing the technical
> component for H. pylori screen tests, eg, Clotest and hp fast?
>
> Thanks in advance?
>
> Diana Goodwin,  HT
> Trenton,  NJ
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 11:49:27 -0600
> From: "Peter A. Takes" <ptakes@stereotaxis.com>
> Subject: Re: CPT code for H. pylori screens
>
> Diana:
>
> If you're looking for individual CPT codes, one place you can check for
> them is
>
> http://otpt.ups.edu/Health_Policy/icd-9andcpt.html
>
> I find this web site useful.  Hope this helps.
>
> Peter
> - --
> Peter A. Takes, Ph.D., RAC
> Director, Clinical & Regulatory Affairs
> STEREOTAXIS, Inc.
> Ph. 1-314-615-6964; Pager 1-314-841-9351
> ptakes@stereotaxis.com
>
> "Goodwin, Diana" wrote:
>
> > Hello, Netters.
> >
> > Does anyone know if there is a CPT code for billing the technical
> > component for H. pylori screen tests, eg, Clotest and hp fast?
> >
> > Thanks in advance?
> >
> > Diana Goodwin,  HT
> > Trenton,  NJ
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 12:06:43 -0600
> From: Katri Tuomala <katri@istar.ca>
> Subject: Re: Calretinin on cytospins revisisted -Reply
>
> Hi!
>
> I would also be interested in this discussion on heat retrieval on
> non-formalin fixed material. By the way Dana, what were your cytospins
> fixed in? Katri.
>
> Katri Tuomala
> Anatomic Pathology
> St.Joseph's Hospital
> Hamilton, Ontario, Canada
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 12:07:06 -0600
> From: "Instrumedics, Inc." <info@instrumedics.com>
> Subject: Re: Knifeholder for the AO cryostat
>
> We have a  knifeholder that Gayle described which can replace the sidearm.
> You can  use a steel blade or a disposable blade holder once installed.
>
> Contact me if interested.
>
> Bernice
> schiller@instrumedics.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 12:07:32 -0600
> From: "Decarli, Terri" <TerDec@northarundel.org>
> Subject: GENTA STAIN
>
> I am looking for a procedure for the Genta Stain sometimes used for H.
> pylori.  I attended the Culling lecture at Providence and it was discussed
> but the procedure was not in the reference material.  Thanks for your
> reply.
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 12:58:06 -0600
> From: "Jeff Crews"<jcrews@organo.com>
> Subject: Re: characterizing cartilage
>
>      If you're interested in the histochemistry of the cartilage, a panel
>      of Alcian blues with different salt concentrations (Critical
>      Electrolyte Concentration technique) w/ and w/o enzyme digestions
> will
>      help characterize the kinds of proteoglycans present. There are also
>      some antibodies available for specific proteoglycans, but I warn you
>      that they're a big pain in the butt to work out.
>         And of course, if you're interested in pathologic calcification of
>
>      the cartilage, you can do Alizarin Red.
>
>                                                                 jc
>
>
> ______________________________ Reply Separator
> _________________________________
> Subject: characterizing cartilage
> Author:  Gary Radice <gradice@richmond.edu> at internet
> Date:    11/04/1999 11:33 AM
>
>
> I have a begun a collaboration with a colleague (a surgeon) who is
> interested in cartilage regeneration. My job is  to determine whether the
> regenerated tissue it looks (histologically) like "real" articular
> cartilage. Most of my experience is with embryonic tissues and with
> muscle.
> I have checked with all the usual suspects (Humason's Animal Tissue
> Techniques, Bancroft and Stevens, AFIP manuals) for standard techniques
> but
> I would also like to correspond with someone who does cartilage routinely
> to get some opinions about protocols that might be particularly diagnostic
>
> for "normality."
>
> Gary P. Radice                        gradice@richmond.edu
> Associate Professor of Biology                804 289 8107 (voice)
> University of Richmond                804 289 8233 (FAX)
> Richmond VA 23173                http://www.science.richmond.edu/~radice
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 14:15:24 -0600
> From: "McCollough, Carol" <CMCCOLLOUGH@dnr.state.md.us>
> Subject: RE: GENTA STAIN
>
> This is a previous response from Bob.  Hope it helps.  Welcome to
> Histonet,
> Terri!
> Regards -
> Carol
> *****************
> Carol B. McCollough, HT(ASCP)
> Diagnostics & Histology Laboratory Manager
> Maryland Department of Natural Resources
> Fisheries Service
> Cooperative Oxford Laboratory
> 904 S. Morris Street
> Oxford, MD 21654
>
>
> From:           RSRICHMOND@aol.com
> Subject:        Re: Genta Stain
> Date:           Wednesday, November  6, 1996 21:57:46 EST
>
> Here it is - I copied it out for somebody who wanted it - certainly
> haven't
> tried it and don't intend to - of academic interest only, in the worst
> sense
> of that abused word.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
> **********************************************
>
> 1. Sections of formalin fixed tissue are cut in the usual fashion,
> deparaffinized, and rehydrated to 	distilled water.
> 2: Sensitize: 1% aqueous uranyl nitrate (uranium nitrate) for 3 minutes.
> 3. Rinse thoroughly in distilled water.
> 4. Silver stain: 1% aqueous silver nitrate in distilled water, beginning
> at
> room temperature, and 	heating in the microwave oven to just below
> the
> boiling point (do not boil).
> 5. Rinse in three changes of distilled water.
> 6. Rinse in two changes of 95% alcohol.
> 7. Rinse in two changes of 100% alcohol.
> 8. Place in 2.5% gum mastic in distilled water for 5 minutes.
> 9. Air dry for one minute.
> 10. Rinse in two changes of distilled water.
> 11. Reduce in REDUCING SOLUTION in a 45oC. water bath for 10 to 15
> minutes,
> until sections 	develop satisfactorily, with black or brown bacteria
> on a
> light yellow background. (Check 	development with a microscope.)
> 12. Rinse thoroughly in distilled water.
> 13. Stain in ALCIAN BLUE pH 2.5 for 10 minutes.
> 14. Rinse in running tap water.
> 15. Stain in Harris hematoxylin for 5 minutes.
> 16. Rinse in running tap water.
> 17. Dip quickly in acid alcohol.
> 18. Rinse in running tap water.
> 19. Blue in 0.25% ammonia water.
> 20. Rinse in running tap water.
> 21. Stain in eosin up to 5 minutes.
> 22. Rinse quickly in tap water.
> 23. Dehydrate, clear, and mount in resin.
>
> REDUCING SOLUTION: 10 mL of 2.5% gum mastic, 25 mL of 2% hydroquinone, 5
> mL
> of absolute alcohol, and 2.5 mL of 0.04% silver nitrate. Do not filter.
>
> Criteria used for the semiquantitative scoring of the density of
> Helicobacter pylori:
>
> 0. None visible.
> 1. Rare scattered isolated organisms, or one or two small clusters in
> surface mucus or in a pit.
> 2. Few scattered organisms. Bacteria can be found without difficulty, and
> many 10x fields 	contain some. They are not crowded or in large
> clumps.
> 3. Organisms uniformly distributed on surface and in pits. Organisms
> usually
> can be seen with no 	difficulty at 4x, and almost every 10x field
> contains some. They are not crowded and do 	not form large clumps.
> 4. Very large numbers of organisms on most mucosal surfaces and pits, with
> bacteria forming an 	almost continuous lining on mucous cells.
> 5. Organisms on entire mucosa, with no free areas.
>
> Robert M. Genta MD. George O. Robason HT(ASCP). David Y. Graham MD.
> (Baylor
> in Houston, at VA center there). Simultaneous visualization of
> Helicobacter
> pylori and gastric morphology: a new stain. Human Pathology, March 1994:
> 25;221-6.
>
> - -----Original Message-----
> From: Decarli, Terri [mailto:TerDec@northarundel.org]
> Sent: Thursday, November 04, 1999 12:59 PM
> To: 'Histonet@pathology.swmed.edu'
> Cc: Decarli, Terri
> Subject: GENTA STAIN
>
>
> I am looking for a procedure for the Genta Stain sometimes used for H.
> pylori.  I attended the Culling lecture at Providence and it was discussed
> but the procedure was not in the reference material.  Thanks for your
> reply.
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 14:36:41 -0600
> From: "Dan Diaz" <dandiaz5@hotmail.com>
> Subject: Gill Hematoxalin II
>
> Hi Histonetters,
>
> Responding to Liz`s request for info on Accra Gill Hematoxalin.  I can get
>
> this product.  Please give me a call at Mercedes Medical, Inc.
> 800-331-2716
> x111,  Ask for Dan Diaz to get pricing and availability.
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 15:46:05 -0600
> From: Amos Brooks <atbrooks@snet.net>
> Subject: Re: HMBR45
>
> Hi,
>     What detection kit are you using? We use a pre diluted DAKO
> antibody, with the LSAB2 system (30 min primary, 10 min secondary, and
> 10 min tertiary) with 20 min HIER epitope retrieval.
> good luck
> Amos Brooks
> HT(ASCP)
>
> joyce judge wrote:
>
> > I am looking for a good antibody and protocol for
> > HMBR45(Melanoma).
> >
> > Joyce Judge
> > Saints Memorial Medical Center
> >
> > =====
> >
> > __________________________________________________
> > Do You Yahoo!?
> > Bid and sell for free at http://auctions.yahoo.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 15:46:32 -0600
> From: "Sarah Christo" <schristo@cvm.tamu.edu>
> Subject: Performic Acid in PFAAB, PAS, Orange G
>
> Dear Netters,
>    I was wondering how hazardous and what precautions need to be made in
> using
> Performic Acid.  This technique mixes formic acid with hydrogen peroxide
> and
> sulfuric acid.  It says to keep the temperature below 25 degrees C.  It
> also
> decomposes in a few hours.  Peracetic Acid (glacial acetic acid, hydrogen
> peroxide and sulfuric acid) can be substituted, would this be any safer???
>  Any help would be appreciated.
> Thanks, Sarah
>
> Sarah Christo, HT (ASCP)
> Texas A&M University
> College of Veterinary Medicine
> Dept. of Vet. Anatomy & Public Health
> Histology Laboratory
> College Station, TX  77868-4458
> schristo@cvm.tamu.edu
> phone (409) 845-3177
> fax (409) 847-8981
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 16:41:07 -0600
> From: Corazon Bucana <bucana@audumla.mdacc.tmc.edu>
> Subject: Decalcification of bone
>
> Is there a commercially available EDTA solution for bone decalcification?
> I
> would appreciate any info on this.
>
> Thanks,
>
> Cora Bucana
> *******************************************************
> Corazon D. Bucana, Ph.D.
> Department of Cancer Biology
> U.T. M.D. Anderson Cancer Center
> 1515 Holcombe Blvd.  Box 173
> Houston, Texas 77030
> Phone: (713) 792-8106
> FAX: (713) 792-8747
> Email:bucana@audumla.mdacc.tmc.edu
> FAX: (713) 792-8747
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 17:03:41 -0600
> From: RSRICHMOND@aol.com
> Subject: RE: GENTA STAIN
>
> Carol B. McCollough, HT(ASCP), Diagnostics & Histology Laboratory Manager,
>
> Maryland Department of Natural Resources, Fisheries Service, Oxford,
> Maryland, posted the Genta stain procedure that I posted in 1996.
> (Goodness,
> Carol, I hope you'res staying out of the way of that awful Pfiesteria
> bug!)
>
> My opinion of this complex and environmentally hazardous procedure hasn't
> changed. I still haven't seen any publication to suggest that this
> procedure
> is clinically any more useful than a simple Giemsa stain.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 18:30:12 -0600
> From: "Jerry Santiago" <santij1@prodigy.net>
> Subject: Pathology Assistants
>
> I was asked my PA to post this message on the histonet concerning the
> pathologists assistant question.
>
> There is a Path. Asst. training program at the University of Maryland in
> Baltimore.  It is a MS level program and will take 2 (two) full years to
> complete.  Info on training programs, a definition of a Path. Asst. and
> other topics can be found on the AAPA web site at:
> www.pathologistsassistants.org
>
> Currently there is no national certification process, although this is in
> development.  The AAPA administers a Membership Exam for election to
> Fellow
> Membership.  This standard is frequently cited by employers.
>
> Sincerely,
> Jerry Phipps,
> Chair, AAPA Board of Trustees
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 19:45:56 -0600
> From: DDittus787@aol.com
> Subject: Re: Calretinin on cytospins revisisted -Reply
>
> I have fixed my cytospins in formalin, alcohol,bouins,have air dried, and
> kept wet, and have gotten equally good results in all.
>                                                       Dana Dittus
>                                                      Abington Memorial
> Hospital
>                                                       Histology Supervisor
>
> P.s. Feel free to call me if you need any method
>        !-215-481-2609
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 21:00:36 -0600
> From: gfenn@bdhinc.com
> Subject: Alcian Blue
>
> Our Supply of Alcian Blue is safe, it is now and will continue to be
> readily
> available.
>
> For distributors please contact :
> in USA  EM Science
> in Canada BDH
> World wide Merck
>
> Regards
> Gordon Fenn
> BDH
>
>
>
>
>
> jim <jim@proscitech.com.au> on 10/31/99 07:45:22 PM
>
> To:   "histonet@pathology.swmed.edu" <histonet@pathology.swmed.edu>
> cc:    (bcc: Gordon Fenn/BDH/EMI/Merck)
> Subject:  Alcian Blue - no more
>
>
>
> Users of Alcian blue should take note and may need to consider
> alternatives:
> An intermediate chemical in the production leading to Alcian Blue is
> hazardous
> and no company is prepared to manufacture this compound. As a consequence
> Alcian Blue, except for any remaining old stock, is no longer available.
> ProSciTech has no more Alcian Blue available.
>
> Our supplier is working with the Staining Commission to produce an
> alternative
> stain that will suit most of the Alcian Blue applications. This may be
> available about April 2000.
> Jim Darley
> ProSciTech                 Microscopy PLUS
> PO Box 111, Thuringowa  QLD  4817  Australia
> Ph +61 7 4774 0370  Fax:+61 7 4789 2313  service@proscitech.com
> Great microscopy catalogue, 500 Links, MSDS, User Notes
>                       www.proscitech.com
>
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 21:19:13 -0600
> From: Amos Brooks <atbrooks@snet.net>
> Subject: Re: Calretinin on cytospins revisisted -Reply
>
> Hello,
>     We have had variable success staining cytospins and smears of various
> fluids without antigen retrieval. We have done lymphoma markers, breast
> cancer
> profiles, cytokeratins and various other stains. The reactivity we have
> seen
> has been primarily dependent on the specimen quality. Air dried smears do
> not
> work well. The specimen should be
> preserved in some way, especially after the thin prep has been done. 70%
> alcohol or Spray Cyte (Addams Scientific).
>     Although reactivity is observed the results of the stains are
> difficult to
> interpret due to an absence of known positive cytology controls. This
> leaves
> quality control virtually impossible. Side by side comparison with tissue
> is
> not quite valid since tissue usually needs target retrieval. Also thin
> preps
> usually do not require as long an
> incubation as tissue sections. If there is anyone who has found a source
> for
> cytology controls please let me know.
> Amos Brooks
> HT(ASCP)
>
> Richard Cartun wrote:
>
> > Do you really need to heat-treat specimens that are not fixed in
> formalin?
> Maybe the gorgeous immunoreactivity you speak of is due to the 37 degree
> primary antibody incubation.  I would appreciate hearing from the Histonet
> community regarding this issue (should non-formalin-fixed specimens be
> heat-treated for optimal immunoreactivity?).
> >
> > R. Cartun
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 22:00:43 -0600
> From: "BB racing" <bbracing@silk.net>
> Subject: Re: Calretinin
>
> I use the Calretinin antibody from Zymed, and for our cytology specimens
> (fixed in 70% alcohol) do not find antigen retreival necessary.  Our
> cytology
> cell blocks are post fixed in Bouins fixative (helps to congeal them into
> a
> solid mass)and these seam to give slightly better results, but again no
> antigen retrieval is necessary.  Tissue samples, (fixed both in 10%
> formalin,
> and formol-acetic acid- alcohol) do not require antigen retrieval to yeild
> excellent results.  I have noticed a slight reduction in sensitivity with
> antigen retrieval with this antibody on our tissue samples. We use two
> methods, our routine technique is to simply place the sections in a coplin
> jar
> of distilled water, which is then place in a beaker of boiling water, and
> let
> them sit in it for ten min once they have reached temperature.  If more
> vigorous antigen retrival is necessary I have been using the Glycerin
> Microwave method.
> Kerry Beebe
> Kelowna Gen Hospital
> Canada
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 22:15:33 -0600
> From: kevin gibbon <gibbowax@uniserve.com>
> Subject: Undecalcified bone
>
> Hi Everyone,
> 	Many thanks to all the replies about the pig tonsils.
>
> I have a question for the hard tissue enthusiasts. I am going to be using
> Technovit 7200 and 4000 in the near future. While I have used JB4 and JB4+
> extensivly I am new to the technovits, could I ask you to send me any
> protocols that you have for them and any tips in their use for both small
> tissue samples and large ones.
>
> Many thanks
>
> Kevin Gibbon
> Wax-it Histology Services
>
>
>
> ----------------------------------------------------------------------
>
> Date: 4 Nov 1999 23:00:47 -0600
> From: Jennifer Saunders <histoed@yahoo.com>
> Subject: slide labeling choices
>
>
> Hello all
> We are looking into different ways to label slides and
> was hoping some of you might have some suggestions. I
> kind of like the idea of using a slide
> labeling/etching system and doing away with labels all
> together. Is this realistic or just fantasy? What are
> your experiences? I have heard that some paper label
> systems are more of a pain as far as entering all the
> information through the computer and so don't really
> save any time. Any and all input welcome.
>
> Thanks
> Jennifer Saunders H.T.
>
>
> =====
>
> __________________________________________________
> Do You Yahoo!?
> Bid and sell for free at http://auctions.yahoo.com
>
>
> Here are the messages received yesterday!




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