I would like to hear the replies also, so please post any responses to the
list.

Robert S. Geske
Research Associate
Center for Comparative Medicine and Department of Pediatrics
Baylor College of Medicine

-----Original Message-----
From:	Jaci Lett [SMTP:jmlett@cid.wustl.edu]
Sent:	Wednesday, November 10, 1999 11:06 AM
To:	Microscopy Listserver; Histonet Listserver
Subject:	B-glucuronidase and carbonyl group localization

Can anyone help me with finding protocols for localization of
B-glucuronidase and carbonyl groups (separate procedures)?

I will be processing mouse cochlea through acetone to Epon-Araldite for 1-4
micron sections and need a protocol that will work after decalcification and
stay throughout processing.  I'm open to protocols that require aqueous
processing (such as glycol methacrylate), but it will still be necessary to
decalcify.

Replies may be sent directly to me.

Thank you for your assistance,

Jaclynn M. Lett, Research Assistant     jmlett@cid.wustl.edu

Fay and Carl Simon Center for the Biology of Hearing and Deafness
Central Institute for the Deaf
818 S. Euclid Ave.
St. Louis, MO  63110

voice:  314-977-0257     fax:  314-977-0030