RE: Adenovirus
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From: | "Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Jane,
We use the Adenovirus antibody from Sera-Lab, Cat# MAS173, Clone H-60.
For FFPE sections, pre-treatment is HIER in Citrate buffer pH6.0, followed
by primary at 1:10,000 for 1hour at RT, detection is LSAB. Have used this
for several years, great results!
Regards
Bryan
> ----------
> From: C & J Haley[SMTP:haley@primary.net]
> Sent: November 14, 1999 5:06 PM
> To: HistoNet Server
> Subject: Adenovirus
>
> We had an older clone of Adenovirus from Biogenex that we were able to
> use on FFPE tissue. It is several years old and started breaking down.
> We ordered the "same" antibody recently and found that they had changed
> clones. Needless to say, the dilution is much lower (from 1:500 to
> 1:10) and it's not as clean. I've had several conversations with tech.
> support, and yes, I know it is a frozen tissue only antibody. (But, so
> was the original clone. Did I mention it was great?) So, I'm looking
> for another source of Adenovirus. Anyone getting good results on FFPE
> tissue? I know this is the fastest way to get lots of great information.
>
> Thanks in Advance,
>
> Jane Haley
> Barnes-Jewish Hospital
> St. Louis, MO
>
>
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