RE: Adenovirus

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From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA> (by way of histonet)
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We use the Adenovirus antibody from Sera-Lab, Cat# MAS173, Clone H-60.
For FFPE sections, pre-treatment is HIER in Citrate buffer pH6.0, followed
by primary at 1:10,000 for 1hour at RT, detection is LSAB. Have used this
for several years, great results!



> ----------
> From: 	C & J Haley[]
> Sent: 	November 14, 1999 5:06 PM
> To: 	HistoNet Server
> Subject: 	Adenovirus
> We had an older clone of Adenovirus from Biogenex that we were able to
> use on FFPE tissue.  It is several years old and started breaking down.
> We ordered the "same" antibody recently and found that they had changed
> clones.  Needless to say, the dilution is much lower (from 1:500 to
> 1:10) and it's not as clean. I've had several conversations with tech.
> support, and yes, I know it is a frozen tissue only antibody.  (But, so
> was the original clone. Did I mention it was great?)  So, I'm looking
> for another source of Adenovirus.  Anyone getting good results on FFPE
> tissue? I know this is the fastest way to get lots of great information.
> Thanks in Advance,
> Jane Haley
> Barnes-Jewish Hospital
> St. Louis, MO

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