HA binding protein

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From:"Brothers, Rachel" <rachel_stock@internet.organo.com> (by way of histonet)
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     Does anyone out there have any experience staininig for
     Hyaluronic Acid Binding Protein or proteoglycans in general?
     I have been trying to stain (human) skin for a panel of proteoglycans
     and HABP is the latest request. The protocol that I have from
     Seikagaku uses a 2 hour incubation in Sigma chondroinase for
     "unmasking" and digestion in hyaluronidase for the negative control.
     Another journal paper that I have doesn't use the chondroitinase step
     at all. I am using formalin-fixed, paraffin sections and have had
     mixed results with the other proteoglycans so far.
     Any tips would be greatly appreciated.

     Rachel Stock

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