B-glucuronidase and carbonyl group localization

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From:Jaci Lett <jmlett@cid.wustl.edu> (by way of histonet)
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Can anyone help me with finding protocols for localization of
B-glucuronidase and carbonyl groups (separate procedures)?

I will be processing mouse cochlea through acetone to Epon-Araldite for 1-4
micron sections and need a protocol that will work after decalcification and
stay throughout processing.  I'm open to protocols that require aqueous
processing (such as glycol methacrylate), but it will still be necessary to

Replies may be sent directly to me.

Thank you for your assistance,

Jaclynn M. Lett, Research Assistant     jmlett@cid.wustl.edu

Fay and Carl Simon Center for the Biology of Hearing and Deafness
Central Institute for the Deaf
818 S. Euclid Ave.
St. Louis, MO  63110

voice:  314-977-0257     fax:  314-977-0030

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