mercury removal before cryotomy

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From:"Hoye, Glenda F. (Fka Hood)" <> (by way of histonet)
To:histonet <>
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I'm looking for a specific reference for a question that is in the NSH
Self-Assessment booklet on Embedding and Sectioning. Number 88 asks about
cutting cryostat sections of B-5 fixed tissue, and the answer is that the Hg
can be removed by iodine, hypo and water before freezing. The question
references are to Bancroft and Stevens, Carson, and Sheehan. But when I look
in those books, I don't find a specific reference -- just info on removal of
Hg in sections already fixed, processed and cut, and that Hg-fixed tissue
would be difficult to cut. Any ideas? I'd love to know that I had just
overlooked something in the reading. My students are asking, and now I'm
very curious as well. Does anyone use this in practical application?
Thanks in advance.
Glenda F. Hoye, BS, HT(ASCP)
Histotechnology Prog Dir, Indiana Univ Sch of Allied Hlth Sci
Indianapolis, IN  46202-5119

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