cutting frozen sections on chicken tendons

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From:Kathy Oprea <koprea@calc.vet.uga.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Hello everyone and Happy Thanksgiving Holidays!!
Does anyone have experience in cutting frozen sections on chicken
tendons? What is the best temperature? we have tried -16 to -20. We
have turn the tendon different angles. We normally cut frozens at 4
microns but have increased the microns to 10. Nothing is working. the
tendon sections continue to be compressed. There are alot of the
samples to be sectioned for a research project. Any suggetions will
be appreciated.
Thank you in advance,
Kathy Oprea
************ Thank you ************
* Kathy Oprea                     *
* University of Georgia           *
* College of Veterinary Medicine  *
* Athens, GA 30602-7388           *
* KOPREA@calc.vet.uga.edu         *
The worker who's never said "Thank
God it's Friday," probably never
really worked.
************************************




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