VZV mAb
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| From: | Richard Cartun <Rcartun@harthosp.org> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
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| Content-Type: | text/plain; charset="us-ascii" |
To: Paul Kwan
We use a mAb (CAT#500113) from Meridian Diagnostics, Inc. [(800) 543-1980]
to localize varicella zoster virus in formalin-fixed, paraffin-embedded
tissues. The mAb is tagged with fluorescein (for direct IF testing in
Microbiology Labs), but works very well in our immunoperoxidase technique
at a dilution of 1:50. Digestion of formalin-fixed tissue sections with
0.4% pepsin (P7000-Sigma) in 0.01 N HCl will improve immunoreactivity.
Richard Cartun
Hartford Hospital
(860) 545-1596
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