Re: demineralized
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
On Mon, 23 Nov 1998 NTT@shcc.org wrote:
> I'm posting this for a fellow researcher. She would like to know if
>anyone has
> a protocol for demineralizing adult rat trachea sections. She thinks
>that the
> Ca++ maybe blocking the antibody.
You don't usually think of the trachea as a "mineralized"
organ. The cartilage rings might be a bit calcified as a
result of some sort of experimentation. In this case any
routine decalcifying procedure would shift the calcium
easily. The idea that calcium ions inhibit antigen-antibody
combination seems novel. There are plenty of ordinary
reasons for failure of an immunohistochemical method!
The first thing to do is make sure you have a control
tissue known to contain the antigen, and stain this
alongside the unknowns. There are other controls too,
especially to eliminate false positive results.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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