Go with Gill 3- it never needs filtering, stains frozens in 20-30 seconds,
counterstains immuno's in 30-60 seconds, clean and crisp chromatin, stable
and non-toxic, can't overstain etc. etc. Six minutes works nice in our H&E
and 1minute in our Pap stain.  Differentiate in 1% HCl in etoh for paraffin
and blue in dilute ammonia, for frozens blue only- no acid, and for cyto's
1 minute and differentiate with  .5% aqueous HCl followed by 1 min water
rinsse to blue. Look for the start of pink runoff in acid baths and stop
immediately with water rinse. Works great every time and lasts 2-sometimes
3 weeks in the staining bath.
Jeff Silverman
Southampton Hospital NY U$A
peptolab@hamptons.com

----------
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> To: HistoNet@pathology.swmed.edu
> Subject: Re: cold hematoxylin
> Date: Wednesday, November 25, 1998 4:27 AM
>
> >Date: Wed, 25 Nov 1998 09:26:54 +1000
> >From: Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au>
> >Subject: Re: cold hematoxylin
> >To: histoNet@pathology.swmed.edu
> >MIME-version: 1.0
> >
> >Histonetter's..out of curiosity,what "homemade" recipe for Haematoxylin
> >would you recommend that doesn't incluse the use of mercuric
> >chloride/oxide??
> >
> >--
> >Bruce Abaloz
> >HISTOLOGIST
> >Department of ZOOLOGY	       *  ph:    +61 3 93446282
> >The University Of Melbourne    *  fax:   +61 3 93447909
> >Parkville Victoria.3052        *  email: b.abaloz@zoology.unimelb.edu.au
> >
> >AUSTRALIA.
> >                    IF YOU WANT TO BE HAPPY......BE!!
> >
>
> Bruce,
> 	Ehrlich, Mayer, Cole, the list goes on and on and on. Ehrlich and
> Mayer are my favourites, saying that, I do lean towards Mayer as it works
> well with a Celestine blue sequence.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>