Re: charged slides

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From:Jeff and Wanda Gray <jefwan@flash.net> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Louise, When we used declere we had better luck using "Gold" slides from
Erie. As I am at home I don't have any cat. numbers etc, but if you
contact Erie, the y will know what you are talking about.  biggest
drawback to these is cost.  Hope this helps, Wanda

Ms Louise Taylor wrote:
>
> Hi histonetters,
>
> We are experiencing a problem with charged slides and "Declere"
> solution from Cellmarque. We find that a large proportion of tissue
> sections (formalin fixed, paraffin embedded) exposed to microwave
> pretreatment with this solution are washing off.
> ("Declere" is purported to be a combination dewaxing, rehydrating and
> antigen retrieval solution)
> Has anyone else had this problem and is there a way to circumvent it?
>
> All comments will be welcome
> many thanks
> Louise Taylor
> SAIMR
> Johannesburg
> South Africa




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