Re: Milli Q H2o
<< Previous Message | Next Message >>
| From: | Louise Burrell <lburrell@pathbox.wustl.edu> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
Dear all: Ditto on the Milli Q, Gayle----I meant to address this last
week---I have been using it for everything, including my water bath for
two years now. When I was being trained in Molecular procedures, I was
introduced to it. I even drink it!!!!! It is the best--even better than
triple distilled from the pharmacy----and cheaper ---in the long haul.
Warmly,
Beezie Burrell
On Wed, 25 Nov 1998, HistoNet Server wrote:
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 02:35:00 -0600
> From: "Mr D. Brotchie" <D.Brotchie@liverpool.ac.uk>
> Subject: Re: tyramide signal amplification kits
>
> We have used the Dako CSA kit successfully. We were able to detect
> GFAP/astrocytes in long term (~ 5 years) NBF-fixed tissue without HIER.
> This was not the case with LSAB or ABC dection techniques as used in our
> hands. We have not tried the Dupont TSA kit.
>
> Regards from Liverpool,
>
> Daniel
>
> ************************
> Daniel Brotchie
> Unit of Ophthalmology
> Department of Medicine
> University of Liverpool
> U.K.
>
> On Mon, 23 Nov 1998 ppyle@notes.mdacc.tmc.edu wrote:
>
> >Hi Histonetters,
> >I wanted to ask if any one had had an success with either the Dako or Du
> >Pont Life Sciences tyramise signal amplification (TSA) kits? We want to
> >increase signal with a research antibody. Anyone had any success??
> >Thanks for your input!
> >
> >Pam Pyle
> >Neuropathology
> >University of Texas M D Anderson Cancer Center
> >713-792-7936
> >ppyle@notes.mdacc.tmc.edu
> >
> >
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 03:15:12 -0600
> From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
> Subject: Re: Declere and Tissue Adhesion
>
> On Mon, 23 Nov 1998, Cell Marque Corporation wrote:
>
> > The Declere protocol calls for the following:
> >
> > A) 3 micron thickness of sections on positively charged slides.
> > B) Dry 1 hour in an incubator which has been properly calibrated at 58 C.
> > C) Use either autoclave (metal pressure cooker with conventional heat
> > source), conventional steamer, or other conventional heat source.
> >
> > I will be happy to address any additional questions.
>
> 1. What does Declere contain?
> 2. How does it work?
> 3. Are there published comparisons (in
> refereed journals) with other procedures?
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 05:01:38 -0600
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: demineralized
>
> >Date: Mon, 23 Nov 1998 17:17:20 -0800 (PST)
> >From: NTT@shcc.org
> >Subject: demineralized
> >To: histonet@pathology.swmed.edu
> >MIME-version: 1.0
> >
> >Hi all,
> >
> >I'm posting this for a fellow researcher. She would like to know if
> >anyone has
> >a protocol for demineralizing adult rat trachea sections. She thinks that
> the
> >Ca++ maybe blocking the antibody. Any reply or shared information (i.e.
> >protocol) would be appreciated. Thanks!!!!
> >
> >Noi
> >
> Noi,
> Is this normal trachea, hyaline cartilage should give no problems.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 05:02:06 -0600
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: Cold Haematoxylin
>
> >Date: Tue, 24 Nov 1998 13:21:02 +0800
> >From: Gerard Spoelstra <spoelstr@numbat.murdoch.edu.au>
> >Subject: Cold Haematoxylin
> >To: histonet@pathology.swmed.edu
> >MIME-version: 1.0
> >
> >Hello.
> >I would appreciate some opinion on a reliable haematoxylin that requires no
> >heat or minimal heat in its preparation. We nearly had a fire when making
> >up the Harris's Haematoxylin recently and are now hunting around for an
> >alternative that does'nt require the use of a bunsen burner. Your
> >suggestions will be apprecitiated!
> >
> >Gerard Spoelstra
> >Histopathology Laboratory
> >Division of Veterinary and Biomedical Science
> >Murdoch Universtity
> >Western Australia
> >
>
> Gerard,
> Brave man with a bunsen. What about Ehrlich, Mayer's with natural
> ripening or Mayer's haemalum. Gill doesn't need heat.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 08:08:48 -0600
> From: "Barry Rittman" <brittman@mail.db.uth.tmc.edu>
> Subject: Re: Cold Haematoxylin
>
>
> Gerald,
> I would suggest and highly recommend the traditional Ehrlich's
> hematoxylin. It takes a little longer to prepare and to stain but has
> excellent keeping qualities and is an excellent stain. If you haveany
> problem finding the formula please let me know.
> Barry
>
>
> At 01:21 PM 11/24/98 +0800, you wrote:
> >Hello.
> >I would appreciate some opinion on a reliable haematoxylin that requires no
> >heat or minimal heat in its preparation. We nearly had a fire when making
> >up the Harris's Haematoxylin recently and are now hunting around for an
> >alternative that does'nt require the use of a bunsen burner. Your
> >suggestions will be apprecitiated!
> >
> >Gerard Spoelstra
> >Histopathology Laboratory
> >Division of Veterinary and Biomedical Science
> >Murdoch Universtity
> >Western Australia
> >
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 08:35:42 -0600
> From: Joyce Friedland <joycefr@frontiernet.net>
> Subject: Re: tissue hazards
>
> Trisha,
>
> Microflex makes a Nitrile glove called Nitron One, which the techs in my
> lab like better than any other they've tried...better fit and not smelly. I
> purchase them through Fisher Sci.
> I'm always amazed at people who reject a product known to be safer for them
> on the basis of something like odor. Do they really prefer to have formalin
> absorbed into their skin???
>
> Joyce
>
> ************************************
> * Joyce Friedland *
> * joycefr@frontiernet.net *
> * www.frontiernet.net/~joycefr *
> ************************************
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 08:41:56 -0600
> From: Rob Geske <rgeske@bcm.tmc.edu>
> Subject: Re: Formaldehyde substitutes
>
> <html><div>Trisha,</div>
> <br>
> <div>Trisha,</div>
> <br>
> <div>Sounds like the reason for the change is noxious formaldehyde fumes.
> Is your laboratory ventilation appropriate and efficient? I would suggest
> investing in better ventilation before making such a drastic change in
> fixation protocol. Is you boss a pathologist? If so, I expect the
> "traditions that are hard to abandon" are actually years of
> professional experience that are predicated on the nuances/artifacts
> which result from formalin fixation. I expect that you have thought
> this through, but I would warn you about throwing the baby out with the
> bath water.</div>
> <br>
> <div>Rob</div>
> <br>
> <br>
> <br>
> <div>At 05:10 PM 11/23/98 -0800, you wrote:</div>
> <div>>Hey Gang,</div>
> <div>></div>
> <div>>I got the boss to consider formaldehyde substitutes. She
> wants to see a</div>
> <div>>"PAPER" on comparisons. I am getting literature
> from vendors, but I would</div>
> <div>>like to get some independent information..a journal is what she
> has in</div>
> <div>>mind. Could you(s) give me a reference or send me a paper
> that would</div>
> <div>>help me with the boss? She's great, but traditions are
> hard to abandon.</div>
> <div>></div>
> <div>>I and my pickled lungs (eyes, sinuses et.) thank you.</div>
> <div>></div>
> <div>>Trisha Emry</div>
> <div>>Orthodontics Research Lab</div>
> <div>>University of Washington</div>
> <div>>Box 357446</div>
> <div>>Seattle, WA 98195</div>
> <div>></div>
> <div>>emry@u.washington.edu</div>
> <div>>(206) 685-8163 fax </div>
> >
> <BR>
>
> <tt><font color="#0000FF"><div align="center">
> Robert S. Geske<br>
> Research Associate<br>
> Center for Comparative Medicine and Department of Pediatrics<br>
> Baylor College of Medicine</font></i></html>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 12:16:16 -0600
> From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
> Subject: Re: demineralized
>
> On Mon, 23 Nov 1998 NTT@shcc.org wrote:
>
> > I'm posting this for a fellow researcher. She would like to know if anyone
> has
> > a protocol for demineralizing adult rat trachea sections. She thinks that
> the
> > Ca++ maybe blocking the antibody.
>
> You don't usually think of the trachea as a "mineralized"
> organ. The cartilage rings might be a bit calcified as a
> result of some sort of experimentation. In this case any
> routine decalcifying procedure would shift the calcium
> easily. The idea that calcium ions inhibit antigen-antibody
> combination seems novel. There are plenty of ordinary
> reasons for failure of an immunohistochemical method!
> The first thing to do is make sure you have a control
> tissue known to contain the antigen, and stain this
> alongside the unknowns. There are other controls too,
> especially to eliminate false positive results.
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 12:16:48 -0600
> From: "Barry, Lilith" <Lilith.Barry@nrc.ca>
> Subject: Beta amyloid stain
>
> Dear colleagues,
> Which would be the best way to visualize Beta amyloid deposition in the rat
> brain. There are few histocemical methods in my Histotechnique book and I
> don't know which one is the most suitable. Or should I do
> immunohstocemistry? If so, which antibody should I use? I have very little
> tissue to work with, so I cannot afford experimenting much. The tissue has
> been fixed with formaldehyde for about 2-3days and are in PBS now.
> I would very much appreciate your help.
> Lilith
> -----------------------------------------
>
> Lilith Ohannessian-Barry
> National Research Council
> Institute of Biological Sciences
> CANADA
> Tel;613-993-6460
> Fax;613-941-4475
> e-mail; lilith.barry@nrc.ca
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 12:17:25 -0600
> From: "Weems, Joyce" <JWEEMS@sjha.org>
> Subject: RE: Medicare Billing
>
> Hi Margarie and all!
> I have been mulling over this billing thing for over a year and am still
> confused!!! I have asked our legal department about it and they are
> confused!!!
>
> Our biggest problem has been with consults, which is what I am trying to
> change. If we send out consults and bill the patient from our
> institution, we often incur more charges when the consulting pathologist
> does additional special stains or immunos. We then can not collect
> those charges, because it has been too long past the date of service.
> Now, granted, the biggest problem is with Medicare, (our census is ~65%
> Medicare!), but insurance companies are also beginning investigations so
> that everything is under scrutiny. The problem is that Medicare charges
> FRAUD instead of error!
>
> I am looking forward to what answers we are going to come with!
>
> HAPPY THANKSGIVING EVERYONE!
> Joyce
>
> >----------
> >From: Hagerty, Marjorie A.[SMTP:mhagerty@emc.org]
> >Sent: Monday, November 23, 1998 8:18 PM
> >To: Histonet
> >Subject: Medicare Billing
> >
> >
> >Hi Everyone,
> >
> >After reading Joyce Weem's explanation concerning medicare billing
> >requirements, I find myself very confused. I am, however, less confused
>than
> >I was before I read it! I am beginning to think that I am making some
> >illegal billing decisions. (Oh, no!)
> >
> >My question is this - Where can I find out more information on this? What
> >can I read?
> >Is anyone willing to explain this to me in a phone conversation? Joyce? Any
> >billing experts?
> >
> >All help and direction will be greatly appreciated. I don't want all
> >reference laboratories to bill us because I know we will lose money. I
>would
> >like to have the reference laboratory bill whenever it is legal.
> >
> >Marg
> >Rancho Mirage, CA, USA
> >mhagerty@emc.org
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 12:18:08 -0600
> From: "Weems, Joyce" <JWEEMS@sjha.org>
> Subject: RE: Who bills Medicare for referral tests
>
> Great explanation, Don. I take it you are talking about pathologist's
> consults as well as such specimens as muscle biopsies, kidney biopsies,
> etc. What happens when the consulting pathologist sends for the paraffin
> blocks and does 5 more immunos that the referring institution can no
> longer bill for? I've been carrying those costs for over a year now,
> sometimes losing as much as $1000 on one consult. I "talked" about this
> in Margorie's post, but it fits well here too!!
> Aren't these challanges fun?!
>
> >----------
> >From: D. Hammer[SMTP:hammerd@u.washington.edu]
> >Sent: Tuesday, November 24, 1998 12:21 AM
> >To: Priscilla
> >Cc: Histonet; J. Fisher; helen shawcroft; Darlene Wood
> >Subject: Re: Who bills Medicare for referral tests
> >
> >Priscilla,
> >
> >When a patient is admitted for a procedure, lets say for example, a
> >Cholestectomy. Medicare has assigned a DRG (Diagnostic Related Group) to
> >that procedure. It pays the Hospital a lump sum for the entire procedure,
> >from the bed, to the surgery, to the lab tests, to the tests referred out
> >to another institution, to the gauze and Kim wipes. It also assumes that
> >any tests done within 72 hours of admit are included, such as CBC's or any
> >other pre surgery testing.
> >
> >When the hospital sends a specimen to a referral lab, they are
> >purchasing tests they don't have inhouse or need further work up. These
> >tests, in Medicare's mind are paid for in the DRG. (just like the Kim
> >Wipes) Medicare does not care how much energy or money you spend on
> >patient care, they only pay what they have set for the DRG. That is the
> >impetus for doing things differently to cost less, and keep the same
> >quality. (quite a challange after awhile, especially improving quality as
> >well)
> >
> >Thus, when a specimen is referred out to another lab, the Hospital has
> >to be billed as, in Medicare's mind, they have paid for the service
> >already and will not pay again.
> >
> >If, in fact, the lab which the specimen was referred to bills Medicare
> >before the orriginating Hospital does, they may pay the lab in error and
> >then disallow payment to the Hospital who will lose the entire payment of
> >the DRG, while the lab collects a small portion for their procedure. This
> >is illegal and is fraud, even if unintentional.
> >
> >I hope this helps to understand why labs who have recieved referral
> >specimens must bill the institution it came from.
> >
> >Specimens referred to another lab from Outpatients can be billed to
> >Medicare. I will just say, that I have decided our Lab will bill the
> >Hospital for all Medicare Pt. referrals, whether inpatient or out
> >patient. It is almost always impossible to determine their status of when
> >the specimen was removed. Some referrals come with historical blocks or
> >slides along with a recent procedure. It could be a mix of inpatient and
> >out patient material.
> >
> >Besides, Medicare is setting up APG's for outpatients which is essentially
> >the same way of payment as the DRG's for inpatients. The same ruling
> >will probably apply shortly there after.
> >
> >By billing the Hospital for both in and out pt. I hope to keep both
> >institutions out of a fraud claim.
> >
> >If anyone has a better or different approach, I would appreciate hearing
> >about it? Confirmation would help ease my mind as well. :)
> >
> >Thanks, Don
> >
> >~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> >Don Hammer, Administrative Director UNIVERSITY OF WASHINGTON
> >Hospital Pathology, Box 356100 MEDICAL CENTER
> >1995 NE Pacific St.
> >Seattle Washington, 98195 ~Where Knowledge Comes To Life~
> >(206) 548-6401 Fax: (206) 548-4928
> >~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> >
> >
> >On Mon, 23 Nov 1998, Priscilla wrote:
> >
> >> I've been wading through the quote from the national intelligence
> >> report about how to bill for referral tests.
> >>
> >> I don't understand why the hospital should be the one to bill Medicare
>when
> >> they will eventually receive a bill from the reference lab for the full
> >> price of the test. Most of the time the reason a test is sent out is
> >> because that particular test is not ordered frequently enough to have the
> >> overhead of keeping reagents required for the test or to justify a
> >> technicians time to run. Hospital laboratorys don't order the testing
> >> whether done in house or referred. A physician orders the tests. Why
> >> should the hospital be put in a position to pay full price on a test that
> >> someone else has ordered, someone else has run and be reeiembursed at a
> >> fraction of the cost of the test, sometimes even lower than it costs
>to run
> >> the test, not even including the cost of packaging raw material to be sent
> >> to the lab? (Of course, I don't think anyone should have to be
>reeiembursed
> >> in that manner, even reference labs.)
> >>
> >>
> >> In the paragraph that talks about the 70/30 rule--how is that percentage
> >> decided? On an average from the previous year? And how would a person
> >> know when that ratio has been exceeded?
> >>
> >> In the paragraph that talks about the lowest of three possible rates: Who
> >> is the carrier lab? What is meant by the national limitation amount and
> >> who sets that? What is meant by median? I believe the provider would be
> >> the referring lab.
> >>
> >> I hope I don't bore with these questions, but a person will never learn if
> >> he or she doesn't ask the questions.
> >>
> >> Cheers! Priscilla in Central Wyoming
> >>
> >>
> >>
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 12:18:50 -0600
> From: gossg@po.lab.ccf.org
> Subject: ATT PEGGY WENK
>
> Hi Peggy,
> I know you're at an institution that has a large tube system & wondered
>if you
>
> could give me any info on the following?
>
> Who is responsible for your in house operational maintenance & trouble
> shooting?
> they're considering using our LIS personnel instead of the main
>hospital
> ITD personnel & they're already getting paged in quite often for
>
> operational trouble shooting.
>
> Can you tell me what the salary range of the techs that care for the
>system or
>
> give me a contact person I can ask?
>
> Thanks
> Any help would be appreciated.
> Gwen
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 12:19:15 -0600
> From: gossg@po.lab.ccf.org
> Subject: MINNESOTA STATE MEETING 99
>
> Can anyone give me the name & address or email of the program chair for the
> Minnesota state meeting April 30 th 1999?
> Thanks
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 12:19:53 -0600
> From: Linda Jenkins <jlinda@ces.clemson.edu>
> Subject: A/O objective
>
> Andi: Are you old enough to remember the old-fashioned hardware store
> where incredible stuff was stacked to the ceilings? Well...I know a
> microscope "shop" like this. It is a real treasure trove -- and, for sure,
> he will have your objective. It is Martin Microscope in Easley,SC. Phone
> is 864-859-2688. He ships all over the world.
> Linda
>
> *********************************
> Linda Jenkins, HT
> Clemson University
> Department of Bioengineering
> Clemson, SC
> **********************************
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 12:20:36 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: cold hematoxylin
>
> Maybe an investment in a stirring hot plate would be safer, we eliminated
> bunsen burners from the lab years ago with fire hazard in mind, and
> have a small magnetic stirrer with heating element. Saved the day!
> Use boiling beads (glass, wash and recycle) to disperse the
> boiling solution and prevent a geyser effect of things boiling over.
> Or a large magnet stirring vigorously, if you don't like the glass beads.
>
> Still love my homemade Harris Hematoxylin with mercuric chloride, but alas
> mercury disposal eliminated this solution from the laboratory and never
>cliked
> the sodium iodate oxidized solution as well. Our overall solution was to
> purchase a commercial hematoxylin that withstands freezing during shipping,
> but not everyone can afford this or likes the hands on approach.
>
> Gayle Callis
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 12:21:00 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: millipore pricing/some help on expenses
>
> The Millipore MilliQ water system is approximately $5000 for the system,
> and if you purchase filters from Millipore, they are $500 a shot! SIGH!
>
> However, Fisher has filters that cost half the price, as a substitute
> for the MilliQ filters, with the same results. Talk to your Fisher Sales rep
> to find out about this, it may say you some precious funds.
>
> Gayle Callis
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 12:21:43 -0600
> From: bakerj@umich.edu (john baker)
> Subject: CryoJane system
>
> Histonetters, I am looking for information from users of the CryoJane
> sectioning system by Instrumedics. I was given the users list but several
> of the numbers are outdated. I work in the Orthopaedic Research Lab at The
> University of Michigan. The cryostat I use is the Hacker/Bright OTF unit.
> I cut mineralized bone biopsys, whole rat mandibles and bones of similar
> size. Sections are 7-10 microns. Immunohistochemistry is carried out now
> on the tissues and eventually insitu hybridization will be done on
> sections. If you use this system and can assist me with your thoughts
> please reply to this message.
>
> Does anyone have the phone number for Patsy Ruegg, if she does not respond
> to this message herself? She is on Instrumedics user's list but the number
> is not correct.
>
> Thanks to all, Happy Thanksgiving. John
>
> John A. Baker
> The University of Michigan
> Orthopaedic Research Laboratories
> Histology Unit
> 400 North Ingalls, G161
> Ann Arbor, MI 48109-0486
> my office 734-936-1635
> lab office 734-763-9674
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 14:25:50 -0600
> From: "Steven E. Slap" <ebs@ebsciences.com>
> Subject: microwave staining
>
> Hi HistoNetters!
>
> i've been out of the country the last two weeks, and missed all the fun
> about microwave processing and staining :-)
>
> However, I really want to reinforce a point- one post gave as a
> direction that the **** solution "can be microwaved for 45 seconds at 70
> percent power." (details changed to protect the guilty)
>
> 70 percent power is not a constant. 70 percent power is not the same
> from one microwave to another. 70 percent power is not the same when the
> magnetron is cold in the morning and warmer later in the day. 70 percent
> power is not the same for a new microwave and one used daily for some
> years. Saying that something should be microwaved at 70 percent power
> tells you absolutely nothing at all.
>
> All microwave procedures should specify either temperatures or output
> wattage or both.
>
> End rant. Ah, I feel better now.
> Steven Slap
>
>
> ********************************
> Energy Beam Sciences, Inc.
> The Laboratory Microwave Company
> http://www.ebsciences.com
> ********************************
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 14:27:57 -0600
> From: slipper@net-gate.com
> Subject: Paraffin squares
>
> Hi, Netters!
>
> Does anyone know of a source for those flat, red paraffin squares that
> are sometimes used to pin muscle biopsies?
>
> - --
> Diana Goodwin, HT
> Trenton, NJ
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 14:56:36 -0600
> From: "M.A. Jennings" <jennings@mayo.edu>
> Subject: Re: pure water system, further info
>
> I agree whole heartly with Gayle about using a consistent water source.
> Fisher carries the nanopure that we use from Barnstead check out:
> http://www.fishersci.com/fb/itv?2..f97.3F.fsc95_18.384..3.9. it has prices
> and a mixture of specific needs. anita
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 14:57:35 -0600
> From: NTT@shcc.org
> Subject: thanks
>
> Hi to all that replied to my demineralizing. You guys are always so
>helpful.
> I will give this to the other researcher. I'll give let you know how it
>turns
> out. Have a happy thanksgiving!!!!!!
>
> Noi
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 14:58:06 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: Instrumedics tape transfer/frozens/bone
>
> We have been using the Instrumedics tape transfer system for frozens,
> and will be using it for undecalcifid murine nasal turbinates. I can
> honestly admit that it is creme de la creme for cryosectioning difficult
> frozen tissues! Lungs do not have to be perfused with anything, but have
> done this with OCT diluted 1:1 with PBS to expand the alveoli via perfusion
> through the trachea (only 1 1/2 mls needed!), snap frozen with dry ice
> isopentane slurry.
>
> The Cryojane is the freezing component of this system, but for bone
> frozen sections, Dodds et al, recommended using (demonstrated in a
> workshop) bone dipped in a 4% PVA solution (Sigma 124,000-186,000)
> and snap frozen in a dry ice/hexane slurry, we substituted this with
> the 2 methylbutane), evaporating solvent off with specimen sitting on
> dry ice. Liquid Nitrogen temps can be too cold & can shatter bone, and
> the Cryojane was too warm. Mount the frozen bone onto a chuck with
> either the PVA or OCT, cover specimen with OCT for sectioning, but with
> the tape transfer, this probably is not necessary. Since bone is so
> hard, I prefer to mount it with 3% methylcellulose which gives rock hard
> holding qualities to reduce chatter during sectioning. Aldrich has this.
>
> We are able to put the device in our particular cryostat without a permanent
> installation, but some cryostats may not permit this, and the component
> that flash polymerizes the polymer with UV light may need to be installed
> and left in the cryostat. A workable situation, in any case.
>
> The slides are now available in 4X, 1X and 0.5X coatings with 4X
> supposedly holding onto the tissue section better than 1X. Personally
> I like the 1X since it is less gooey to handle, but all in all, I
> highly recommend the system. A tidge more expensive, but I waste so
> few slides since they are all usually good for IHC work, it breaks
> about even in the long run.
>
> The only thing I found to be annoying is the polymerized layer tends to
> look bubbly with an aqueous mounting media, but solved that with Biomeda's
> crystal mount or any equivalent (Shandon has one, Biogenex, etc) a liquid
> coverslip barrier for AEC and solvent sensitive chromogens then you can
> add a permanent coverslip.
>
> I have sectioned mink skin that basically contains pure liquid form
> lipid, worst stuff I ever saw! and the section was perfect, retaining
> hair follicles usually lost with regular frozens since the cryostat
> could not be set cold enough to solidify that yucky lipid content.
> I takes just a few minutes to become proficient at using it, I taught
> a grad student in 10 minutes! Serial frozen sections are a snap
>
> I have seen it demonstrated with larger bone sections but takes a good
> sharp tungsten carbide knife, no matter what! DDK is a good source
> for these.
>
> Now I hope Bernice sends me my commission for Christmas, the tape
> transfer device was an excellent, timely investment and if the lab burns
> down, I grab my purse, the Instrumedics device and run for the door! Is this
> a hard sell/recommendation or what?!?
>
> Happy thanksgiving to all!
> Gayle Callis
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 14:58:34 -0600
> From: "D. Hammer" <hammerd@u.washington.edu>
> Subject: Re: ATT PEGGY WENK
>
> Interested here as well on Tube systems and if there are any out their
> using robots to deliver specimens from surgery to pathology. I have
> suggested our consultant on tube design to look into this but would like
> to offer some assistance.
>
> Thanks, Don
>
>
>
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Don Hammer, Administrative Director UNIVERSITY OF WASHINGTON
> Hospital Pathology, Box 356100 MEDICAL CENTER
> 1995 NE Pacific St.
> Seattle Washington, 98195 ~Where Knowledge Comes To Life~
> (206) 548-6401 Fax: (206) 548-4928
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>
>
> On Tue, 24 Nov 1998 gossg@po.lab.ccf.org wrote:
>
> > Hi Peggy,
> > I know you're at an institution that has a large tube system & wondered if
> you
> > could give me any info on the following?
> >
> > Who is responsible for your in house operational maintenance & trouble
> shooting?
> > they're considering using our LIS personnel instead of the main
>hospital
> > ITD personnel & they're already getting paged in quite often for
>
> > operational trouble shooting.
> >
> > Can you tell me what the salary range of the techs that care for the system
> or
> > give me a contact person I can ask?
> >
> > Thanks
> > Any help would be appreciated.
> > Gwen
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 14:59:04 -0600
> From: LINDA MARGRAF MD <LMARGRAF@childmed.dallas.tx.us>
> Subject: San Diego position
>
> Here's a message Melody was having trouble posting. Please respond to her or
> her institution's HR office . Thanks
>
>
> EMPLOYMENT OPPORTUNITY FOR BIOTECH COMPANY IN SAN DIEGO:
>
>
> PharMingen, a rapidly growing biotech company engaged in the development,
> manufacture & marketing of quality biomedical research products, has the
> following position available:
>
> QC ASSOCIATE I, IMMUNOHISTOCHEMISTRY (#424) Min 1-2 yrs lab exp incl min 1 yr
> in Immunohistochemistry req. Exp w/sectioning human & animal tissue using
> Cryostat & Microtome req., Sliding Microtome exp pref. Resp incl testing
> antibodies that req IHC appls, maintaining tissue banks & slide inventory &
> collecting fresh tissue samples. Prev animal handling exp pref. B.S. in
> biology/related science req. or equiv work exp & education.
>
>
> PharMingen offers a competitive compensation & benefits package, including a
> matching 401(k) plan & a stimulating work environment. Please send resume &
> salary requirements indicating the position of interest by referencing the
> job number to:
>
> Human Resources, PharMingen, 10975 Torreyana Road, San Diego, CA 92121 or fax
> to (619) 812-8888. Resumes without salary requirements may not be considered.
> EOE
> Please visit our WEB site at www.pharmingen.com
> PHARMINGEN
> "A Becton Dickinson Company"
>
>
>
> Melody Ricci, IHC-QC Supervisor
> (619) 812-8800 Ext.3346
> PharMingen, San Diego
> (A Becton-Dickinson Company)
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> !
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> !
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 16:01:41 -0600
> From: "Jeff Silverman" <peptolab@hamptons.com>
> Subject: Re: Grossing
>
> Re: grossing by HT's
> I have been regularly grossing for eight years for the pathologists in my
> institution doing eveything from endoscopic biosies to hysterectomies,
> lumpectomies, nephrectomies and colectomies and mastectomies, as well as
> regrosses. All big cases and any skin lesion excision or anything with
> complicated margins gets shown to the doc before or during the dissection.
>
>
> There has been no formal credentialling by any hospital committee although
> every day I interact with the surgeons and clinicians in this capacity as
> well as lead histotech. Twenty five years studying surgical pathology in
> community hospital labs and at meetings make me well suited for this task.
> It is we the mighty histotechs who must ultimately demonstrate the lesions
> at the embedding and microtomy centres so who better to examine these
> things grossly.I always cut thin sections. I only wish the AAPA had an
> alternate pathway or a subcategory such as "assistant in surgical
> pathology". Why? I don't do autopsies, surgicals only!
>
> CLIA says if I did a good job before 1995 or ?1996, I don't need an
> associates degree to continue- thank God. I haven't been grossing for a
> year though, the boss is too paranoid. My pathologist, who is going private
> in 1999 and wants me to leave my union job and work for him for
> "piecework", prefers to do it himself now and is ostracizing me to scare me
> into his union busting- my hospital claims losses of 58 million dollars
> over the last three years and is also trying to weaken the unions with
> contract talks at a standstill. They just merged with the two other small
> regional hospitals out here in Suffolk, Long Island, NY and the three and
> all their unions are enlocked in a classic labor/ management struggle and
> I'm in the middle. Mine is the first job they're going to try to
> subcontract to an idenpendent contractor- the pathologist. I've got
> marketable skills, I think, so I'm enjoying the ride and just trying to
> hang on- if not just for the movie script.
>
> Jeff Silverman
> peptolab@hamptons.com
> Southampton Hospital NY USA
> - ----------
> > From: Robin K Ryan <ryanrk@gnv.fdt.net>
> > To: HistoNet@Pathology.swmed.edu
> > Subject: Grossing
> > Date: Monday, November 23, 1998 7:06 PM
> >
> > Hi Don,
> >
> > At our institution the pathologists do all of the "grossing". There are
> > five histotechnologist and two pathologists. Several of us have
> experience
> > in grossing but they prefer to do it themselves, although, if a biopsy
> > comes in (endo., cx. bx., liver, etc.) we do put the gross description on
> > the surgical requisition, place the tissue in the cassette and process it
> > rather than holding it until the next day. The pathologists do their
> gross
> > description based on what we write on the requisition and what they see
> on
> > the slides. The only experience we have is what some other trained
> person
> > taught us-no formal schooling for grossing. Hope this helps.
> >
> > Kaye Ryan
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 16:02:35 -0600
> From: "Jeff Silverman" <peptolab@hamptons.com>
> Subject: Re: antibody or ? search
>
> Cindy,
> CD68 is lysosomal glycoprotein and should stain immature granulocytes. I
> don't know if there is species homology or even a canine CD68 antibody, but
> maybe someone does.
> peptolab@hamptons.com
> Jeff Silverman
>
> - ----------
> > From: Cindy Farman <cfarman@sierrabiomedical.com>
> > To: 'Histonet' <Histonet@Pathology.swmed.edu>
> > Subject: antibody or ? search
> > Date: Monday, November 23, 1998 4:54 PM
> >
> > Hi everyone,
> >
> > Okay, here's a tough one. I might just have to give a prize out if
> someone
> > can help me with this. I trying to figure out a way to detect immature
> > granulocytes in formalin-fixed, paraffin-embedded dog liver.
> >
> > Cindy Farman
> > Sierra Biomedical, Inc.
> > Sparks, NV
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 16:03:35 -0600
> From: "D. Hammer" <hammerd@u.washington.edu>
> Subject: RE: Medicare Billing
>
> Joyce,
>
> In a perfect world, the consulting pathologist will get permission from
> the refering institution to run additional special or immunos. As a
> matter of fact,the consultant needs to have a record of the order from
> your institution in order to be "legal" in their billing. (if they bill
> insurance companies)
>
> We have the same problem here, but more so, we are remiss in getting the
> permission from the refering institution and giving them info to bill
> with. We do alot of direct insurance billing as opposed to billing the
> institution, but that will change with the Medicare patients. We are
> working on the staff getting permission to run additionaals.
>
> Don't you think, as I do, that NSH should have Dennis Padget (Padget
> Associates) give a half or all day workshop on Pathology billing. People
> would be amazed at how complex it is and how many things are not done
> correctly. My god, there are still people billing for slides and/or
> blocks.
>
> Don
>
>
>
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Don Hammer, Administrative Director UNIVERSITY OF WASHINGTON
> Hospital Pathology, Box 356100 MEDICAL CENTER
> 1995 NE Pacific St.
> Seattle Washington, 98195 ~Where Knowledge Comes To Life~
> (206) 548-6401 Fax: (206) 548-4928
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>
>
> On Tue, 24 Nov 1998, Weems, Joyce wrote:
>
> > Hi Margarie and all!
> > I have been mulling over this billing thing for over a year and am still
> > confused!!! I have asked our legal department about it and they are
> > confused!!!
> >
> > Our biggest problem has been with consults, which is what I am trying to
> > change. If we send out consults and bill the patient from our
> > institution, we often incur more charges when the consulting pathologist
> > does additional special stains or immunos. We then can not collect
> > those charges, because it has been too long past the date of service.
> > Now, granted, the biggest problem is with Medicare, (our census is ~65%
> > Medicare!), but insurance companies are also beginning investigations so
> > that everything is under scrutiny. The problem is that Medicare charges
> > FRAUD instead of error!
> >
> > I am looking forward to what answers we are going to come with!
> >
> > HAPPY THANKSGIVING EVERYONE!
> > Joyce
> >
> > >----------
> > >From: Hagerty, Marjorie A.[SMTP:mhagerty@emc.org]
> > >Sent: Monday, November 23, 1998 8:18 PM
> > >To: Histonet
> > >Subject: Medicare Billing
> > >
> > >
> > >Hi Everyone,
> > >
> > >After reading Joyce Weem's explanation concerning medicare billing
> > >requirements, I find myself very confused. I am, however, less confused
> than
> > >I was before I read it! I am beginning to think that I am making some
> > >illegal billing decisions. (Oh, no!)
> > >
> > >My question is this - Where can I find out more information on this? What
> > >can I read?
> > >Is anyone willing to explain this to me in a phone conversation?
>Joyce? Any
>
> > >billing experts?
> > >
> > >All help and direction will be greatly appreciated. I don't want all
> > >reference laboratories to bill us because I know we will lose money. I
> would
> > >like to have the reference laboratory bill whenever it is legal.
> > >
> > >Marg
> > >Rancho Mirage, CA, USA
> > >mhagerty@emc.org
> > >
> > >
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 16:04:03 -0600
> From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
> Subject: RE: antibody or ? search
>
> Cindy:
>
> About the most reliable technique is the enzyme histochemical method:
> Leder's Napthol AS-D chloroacetate esterase (CAE). The enzyme remains
> detectable after routine formalin fixation and paraffin embedding. The stain
> is (+) for mast cells and granulocytes. It is (-) for other lymphoid cells.
>
> Immunohistochemically, immature canine myeloid cells are CD32(-),
> CD64(weakly +) and with maturation, as in humans, the CD64 surface antigen
> is lost and CD32 is gained. A polyclonal myeloperoxidase may also work on
> canine tissue.
>
> Eric Kellar
> Histology/Immunohistochemistry
> University of Pittsburgh Medical Center
>
> ----------
> From: Cindy Farman [SMTP:cfarman@sierrabiomedical.com]
> Sent: Monday, November 23, 1998 4:55 PM
> To: 'Histonet'
> Subject: antibody or ? search
>
> Hi everyone,
>
> Okay, here's a tough one. I might just have to give a prize out if
> someone
> can help me with this. I trying to figure out a way to detect
> immature
> granulocytes in formalin-fixed, paraffin-embedded dog liver.
>
> Cindy Farman
> Sierra Biomedical, Inc.
> Sparks, NV
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 16:04:30 -0600
> From: "Hagerty, Marjorie A." <mhagerty@emc.org>
> Subject: Billing/Consults,etc.
>
>
> <<Don't you think, as I do, that NSH should have Dennis Padget (Padget
> Associates)>>
>
> Who is this guy Don? I am very interested.
> Thanks,
> Marg
> EMC, Rancho Mirage, CA
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 16:05:52 -0600
> From: "Heather Waddel" <haw@srv4.med.ed.ac.uk>
> Subject: vacuum baths
>
> Hi Histonetters
> Does anybody know of a U.K. supplier of vacuum embedding baths?
> Does anybody have a vacuum embedding bath which is surplus to
> requirements? Perhaps we could purchase it.
> Hope somebody can help.
> Heather
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 16:10:02 -0600
> From: cmcorp <cmcorp@cellmarque.com>
> Subject: Re: Declere and Tissue Adhesion
>
> Dear Patsy,
>
> Have you tried other types of tissue with Declere? Did you have any tissue
> adhesion problems with these? In respect to bone and/or cartilage, do you
> experience tissue adhesion problems with other pretreatment methods?
>
> Generally, with bone, the problem is that the sections are often cut too
> thickly since it may be more difficult to cut 3 - 4 micron. This is
> especially true if there is insufficient decalcification. In respect to
> normal cartilage, decal is not necessary. Only in the circumstance where
> you have abnormal cartilage with calcium deposits would you decalcify. Like
> bone, cartilage sections are often cut too thickly. It may help to dry a
> thicker section longer but only up to a point. Generally as you get much
> above 4 micron thickness you will experience some degree of tissue adhesion
> problems.
>
> I would be happy to discuss this further. It would be helpful to know the
> answers to some of my questions.
>
> Regards,
> Paul Ardi
> 800-665-7284
>
>
>
>
> At 09:56 PM 11/23/98 -0700, you wrote:
> >just tried decleare for the first time on bone and things fell off
> >selectively as usual. the cartilage fell off, the bone stayed on, i
> >used a steamer rather than microwave which is supposed to be more
> >gentle, i was not impressed. all the rules were followed for overnight
> >drying on charged slides, etc.
> >patsy ruegg
> >
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 16:10:29 -0600
> From: "Ian Barclay" <koira@clara.net>
> Subject: Re:
>
> Can you help me as I being told to not send ENCLOSURES!!!!!! and and am only
> filling in the subject line as unsubscribe. There must be something in my
> format which is causing this anomaly
>
> Thanking you in anticipation
>
>
> IGB
>
> - -----Original Message-----
> From: HistoNet Server <HistoNet@Pathology.swmed.edu>
> To: Ian Barclay <koira@clara.net>
> Date: 22 November 1998 07:18
>
>
> >Please do not send ENCLOSURES!!!!!!
> >
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 16:12:15 -0600
> From: <don.luke@SGMC.ORG>
> Subject: tube systems
>
> We have a tube system throughout our hospital and it has been determined
> that sending pathology specimens through the tube is unacceptable. This
> is because of the Formalin. A spilled and/or leaking container would
> force formaldehyde fumes all over the hospital.
>
> Don Luke, HT (ASCP)
> SOUTH GEORGIA MEDICAL CENTER
> DEPARTMENT OF PATHOLOGY
> 2501 N. Patterson St
> VALDOSTA, GA 31603
> PHONE (912) 259-4830
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:13:58 -0600
> From: broche@wlgore.com
> Subject: Counterstain
>
> Histonetters:
>
> We are "staining" frozen sections of B-gal. cells to end up with a
> turquoise-blue reaction product. The color is soluble in alcohols and
> clearants. We use crystal mount to permanently mount. We are looking for
> a counterstain that will not obscure the turquoise-blue color and will not
> leach out into the aqueous mounting media. So far our hematoxylin (Mayers)
> is too dark, and neutral and nuclear fast red bleed out into the mountant.
> Anyone have any suggestions? I sort of remember discussions of this nature
> in the past but , of course, didn't archive them at the time.
>
> Thanks for the help,
>
> Beth Roche
> Gore Hybrid Technologies
> Flagstaff, AZ
> broche@wlgore.com
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:15:19 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: Paraffin squares
>
> Diana,
> I am not familiaar with those. I make paraffin sqaures for pinning
> specimens with my embedding media. At the NSH in SLC I took a course from
> Barbara Munch and she used cork. Hope this helps.
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
> > ----------
> > From: Diana Goodwin[SMTP:slipper@net-gate.com]
> > Sent: Tuesday, November 24, 1998 12:29 PM
> > To: Histo-Net
> > Subject: Paraffin squares
> >
> > Hi, Netters!
> >
> > Does anyone know of a source for those flat, red paraffin squares that
> > are sometimes used to pin muscle biopsies?
> >
> > --
> > Diana Goodwin, HT
> > Trenton, NJ
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:15:43 -0600
> From: Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au>
> Subject: Re: cold hematoxylin
>
> Histonetter's..out of curiosity,what "homemade" recipe for Haematoxylin would
> you recommend that doesn't incluse the use of mercuric chloride/oxide??
>
> - --
> Bruce Abaloz
> HISTOLOGIST
> Department of ZOOLOGY * ph: +61 3 93446282
> The University Of Melbourne * fax: +61 3 93447909
> Parkville Victoria.3052 * email: b.abaloz@zoology.unimelb.edu.au
>
>
> AUSTRALIA.
> IF YOU WANT TO BE HAPPY......BE!!
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:16:33 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: Cold Haematoxylin
>
> Gerard,
> I know this may seem extravagant to some but I oredr hematoxlyin
> and Eosin from Richard-Allen. Great reliable product and they have
> always if I have any concerns. Have never had any problems.
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
> > ----------
> > From: Gerard Spoelstra[SMTP:spoelstr@numbat.murdoch.edu.au]
> > Sent: Monday, November 23, 1998 10:21 PM
> > To: histonet@Pathology.swmed.edu
> > Subject: Cold Haematoxylin
> >
> > Hello.
> > I would appreciate some opinion on a reliable haematoxylin that
> > requires no
> > heat or minimal heat in its preparation. We nearly had a fire when
> > making
> > up the Harris's Haematoxylin recently and are now hunting around for
> > an
> > alternative that does'nt require the use of a bunsen burner. Your
> > suggestions will be apprecitiated!
> >
> > Gerard Spoelstra
> > Histopathology Laboratory
> > Division of Veterinary and Biomedical Science
> > Murdoch Universtity
> > Western Australia
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:17:00 -0600
> From: "Weems, Joyce" <JWEEMS@sjha.org>
> Subject: Working Amounts
>
> When you are trying to meet CAP, OSHA and what ever else requirements,
> what is considered a "working amount" of flammable liquid (such as
> xylene or alcohol), that can be out of the flammable cabinet and on the
> counter or under the hood? Thanks, Joyce!!!
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:19:43 -0600
> From: "Mick Rentsch" <ausbio@nex.com.au>
> Subject: Re: millipore pricing/some help on expenses
>
> Dear Gayle,
> you might like to see if you can source filters from "CUNO" they are US
> based and are just about the biggest water purification manufacturers
> worldwide, they also make the filters for other companies including for
> Millipore. I've changed over to Cuno Filters here in Aussieland and they
> only cost me about $50 ea. for my MilliRO system. I buy them through my
> local Irrigation Farm Supplies, because Cuno Aust. will only sell through
> Distributors or to Manufacturers.
> regards Mike Rentsch
> - -----Original Message-----
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
> Date: Wednesday, 25 November 1998 6:37
> Subject: millipore pricing/some help on expenses
>
>
> >The Millipore MilliQ water system is approximately $5000 for the system,
> >and if you purchase filters from Millipore, they are $500 a shot! SIGH!
> >
> >However, Fisher has filters that cost half the price, as a substitute
> >for the MilliQ filters, with the same results. Talk to your Fisher Sales
> rep
> >to find out about this, it may say you some precious funds.
> >
> >Gayle Callis
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:20:10 -0600
> From: "Mick Rentsch" <ausbio@nex.com.au>
> Subject: All purpose Haematoxylin Cold Prep.
>
> Dear Gerald,
> if you want a strong Haematoxylin with the same dye content as Harris and
> non-toxic to boot, then try Lillie-Mayer Haematoxylin. The recipe is in
> "Histologic Technic & Practical Histochemistry" 4th Ed. by Prof.RD Lillie
> and Harold Fulmer p.2004-208. This text is of such bibical proportions and
> trivia inclusions that it is a must for every Histo Lab, and despite it's
> age is ever appropriate.
> It has up to a five year shelf life esp. if you only use Sodium Iodate at
> the rate of 0.2g/Litre. The incorporation of Glycerol as a stabiliser also
> prevents further oxidation and acts as an antifreeze as well.The
> incorporation of Glacial acetic acid is something you will have to control
> or even eliminate depending upon how regressive you wish to use the stain,
> and also depending upon what additional structures you wish to demonstrate
> viz. Mucin and elastin. The recipe instructions emphasise that heat must not
> be used- so you have a winner! Besides which when you plunge your flask of
> boiling harris into water you always run the risk of loosing the lot with a
> heat stress fracture, not to mention the risk of cutting or burning
> yourself.
> Merck Aust. also market a Non-toxic Harris Haematoxylin that performs
> similarly.
> Hope this is of practical use to you,
> Regards Mike Rentsch (Victoria)
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:23:19 -0600
> From: "D. Hammer" <hammerd@u.washington.edu>
> Subject: Re: Billing/Consults,etc.
>
>
> Marg,
>
> He has a firm dedicated to Consulting on Pathology Billing. Altho we have
> done a pretty good job here with the billing "mess" when we had him in a
> few years ago, he helped us identify some good changes.
>
> Most revenue increases will be on the Pro Fee side, but then again,
> Technical fees are usually so much higher than one collects, they will
> improve as well.
>
>
> Don
>
>
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Don Hammer, Administrative Director UNIVERSITY OF WASHINGTON
> Hospital Pathology, Box 356100 MEDICAL CENTER
> 1995 NE Pacific St.
> Seattle Washington, 98195 ~Where Knowledge Comes To Life~
> (206) 548-6401 Fax: (206) 548-4928
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>
>
> On Tue, 24 Nov 1998, Hagerty, Marjorie A. wrote:
>
> >
> > <<Don't you think, as I do, that NSH should have Dennis Padget (Padget
> > Associates)>>
> >
> > Who is this guy Don? I am very interested.
> > Thanks,
> > Marg
> > EMC, Rancho Mirage, CA
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:25:20 -0600
> From: "Hinton, Sandy" <sahinton@utmb.edu>
> Subject: Surgical Pathology Requisitions
>
> We are currently in the process of revising our Surgical Pathology
> requisitions.
> Currently the requisition that we are using is generic.
> Some of our Pathologists would like to have service specific
> requisitions(Example:GYN,GI). The other suggestion is to have a service
> specific worksheet which would be attached to the Surg Path requisition.
> I would appreciate input on what you're doing in other hospital facilities.
> Thanks
> University of Texas Medical Branch at Galveston
> Sandy Hinton
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:26:05 -0600
> From: lpwenk@mail.netquest.com
> Subject: Re: Medicare Billing
>
> D. Hammer wrote:
> >
> > Joyce,
> >
> > In a perfect world, the consulting pathologist will get permission from
> > the refering institution to run additional special or immunos. As a
> > matter of fact,the consultant needs to have a record of the order from
> > your institution in order to be "legal" in their billing. (if they bill
> > insurance companies)
> >
> > We have the same problem here, but more so, we are remiss in getting the
> > permission from the refering institution and giving them info to bill
> > with. We do alot of direct insurance billing as opposed to billing the
> > institution, but that will change with the Medicare patients. We are
> > working on the staff getting permission to run additionaals.
> >
> > Don't you think, as I do, that NSH should have Dennis Padget (Padget
> > Associates) give a half or all day workshop on Pathology billing. People
> > would be amazed at how complex it is and how many things are not done
> > correctly. My god, there are still people billing for slides and/or
> > blocks.
>
> WHY NOT HAVE SOMEONE GIVE A WORKSHOP?!?!?!?
>
> YES, I AM SHOUTING!!! I NEED THIS WORKSHOP, AND OBVIOUSLY,
> SO DO A LOT OF OTHER PEOPLE.
>
> Application deadline for the 1999 NSH S/C is Dec. 1, so
> he'll have to work fast. (Or anyone else who would be
> willing to give this.)
> >
> > Don
> >
> >
> >
> > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> > Don Hammer, Administrative Director UNIVERSITY OF WASHINGTON
> > Hospital Pathology, Box 356100 MEDICAL CENTER
> > 1995 NE Pacific St.
> > Seattle Washington, 98195 ~Where Knowledge Comes To Life~
> > (206) 548-6401 Fax: (206) 548-4928
> > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> >
> > On Tue, 24 Nov 1998, Weems, Joyce wrote:
> >
> > > Hi Margarie and all!
> > > I have been mulling over this billing thing for over a year and am still
> > > confused!!! I have asked our legal department about it and they are
> > > confused!!!
> > >
> > > Our biggest problem has been with consults, which is what I am trying to
> > > change. If we send out consults and bill the patient from our
> > > institution, we often incur more charges when the consulting pathologist
> > > does additional special stains or immunos. We then can not collect
> > > those charges, because it has been too long past the date of service.
> > > Now, granted, the biggest problem is with Medicare, (our census is ~65%
> > > Medicare!), but insurance companies are also beginning investigations so
> > > that everything is under scrutiny. The problem is that Medicare charges
> > > FRAUD instead of error!
> > >
> > > I am looking forward to what answers we are going to come with!
> > >
> > > HAPPY THANKSGIVING EVERYONE!
> > > Joyce
> > >
> > > >----------
> > > >From: Hagerty, Marjorie A.[SMTP:mhagerty@emc.org]
> > > >Sent: Monday, November 23, 1998 8:18 PM
> > > >To: Histonet
> > > >Subject: Medicare Billing
> > > >
> > > >
> > > >Hi Everyone,
> > > >
> > > >After reading Joyce Weem's explanation concerning medicare billing
> > > >requirements, I find myself very confused. I am, however, less confused
> than
> > > >I was before I read it! I am beginning to think that I am making some
> > > >illegal billing decisions. (Oh, no!)
> > > >
> > > >My question is this - Where can I find out more information on this?
>What
> > > >can I read?
> > > >Is anyone willing to explain this to me in a phone conversation? Joyce?
> Any
> > > >billing experts?
> > > >
> > > >All help and direction will be greatly appreciated. I don't want all
> > > >reference laboratories to bill us because I know we will lose money. I
> would
> > > >like to have the reference laboratory bill whenever it is legal.
> > > >
> > > >Marg
> > > >Rancho Mirage, CA, USA
> > > >mhagerty@emc.org
> > > >
> > > >
> > >
> > >
>
> - --
> Peggy A. Wenk, HTL (ASCP)
> Anatomic Pathology
> Wm. Beaumont Hospital
> 3601 W. 13 Mile Rd.
> Royal Oak, MI 48073-6769
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:26:38 -0600
> From: lpwenk@mail.netquest.com
> Subject: Re: ATT PEGGY WENK
>
> gossg@po.lab.ccf.org wrote:
> >
> > Hi Peggy,
> > I know you're at an institution that has a large tube system & wondered if
> you
> > could give me any info on the following?
>
> Sorry to disappoint. Anatomic Pathology does NOT have a
> tube system. We have surgical pathology assitants/aides
> and OR aides who run around, dispersing specimens.
>
> Clinical Pathology does have some tube systems. If you are
> still interested, I can find out about it on the
> clin path side.
>
> Let me know.
> >
> > Who is responsible for your in house operational maintenance & trouble
> shooting?
> > they're considering using our LIS personnel instead of the main
> hospital
> > ITD personnel & they're already getting paged in quite often for
> > operational trouble shooting.
> >
> > Can you tell me what the salary range of the techs that care for the system
> or
> > give me a contact person I can ask?
> >
> > Thanks
> > Any help would be appreciated.
> > Gwen
>
> - --
> Peggy A. Wenk, HTL (ASCP)
> Anatomic Pathology
> Wm. Beaumont Hospital
> 3601 W. 13 Mile Rd.
> Royal Oak, MI 48073-6769
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 18:27:20 -0600
> From: Rod Green <rod.green@cwixmail.com>
> Subject: Re: MINNESOTA STATE MEETING 99
>
> Hi Gwen,
>
> The person that you want is Shea Merrill at:
>
> Medical Institute of Minnesota
> 5503 Green Valley Drive
> Bloomington, MN 55437
> Ph: 612.844.0064 x216
> Fax: 612.844.0472
>
> Have a good turkey day,
>
> Rod Green
>
> gossg@po.lab.ccf.org wrote:
>
> > Can anyone give me the name & address or email of the program chair for the
> > Minnesota state meeting April 30 th 1999?
> > Thanks
>
>
>
> ----------------------------------------------------------------------
>
> Date: 24 Nov 1998 20:35:24 -0600
> From: DayDawning@aol.com
> Subject: Re: tube systems
>
> My understanding of the tube systems, or the way they were explained it to
> me, is: the formalin eats away at the stainless steel.
>
>
> D isclaimer-- I left the clinical situation before any resolution occurred
> on our system.
>
> Dawn
>
>
> Here are the messages received yesterday!
>
>
<< Previous Message | Next Message >>