Re: IHC Books
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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On Wed, 18 Nov 1998 Cindipqr@aol.com wrote:
> Would someone please recommend some good IHC books for me? Thanks
Polak, Julia M. & Van Noorden, Susan "Immunohistochemistry"
2nd ed. 1997. Bios Scientific Publishers, Oxford. 160 pages.
This excellent little book explains the mechanisms and tells you
what to do. Very clearly written, and better than some bigger
books on the subject. It's one of the Royal Microscopical
Society's series of monographs: a paperback that's cheap only
in its price.
The DAKO company has a free booklet that's very good for practical
instructions, but it's a bit thin on the "theory." When you do
an immuno stain it's very important to _understand_ the reason
for every step. If you follow instructions without thinking and
reasoning it won't be long before you get a failure (false positive
or false negative), and you will not know how to find out what's
wrong. (Looking back over this paragraph it looks like a criticism
of the DAKO booklet. It isn't; the booklet is very good, but it
assumes some knowledge of the principles of immunohistochemistry.)
The enquirer gives the impression that she must learn about it from
scratch. That's why I recommend a small book that explains the whys
as well as the howtos. There are plenty of big book that cover this
field, and you will probably get many replies!
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
E-mail: kiernan@uwo.ca
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